Chimeric antigen receptors containing a chlorotoxin domain
AssigneeCity of Hope
InventorsMichael Barish, Christine E. Brown, Stephen J. Forman, Dongrui Wang
Life of this patent
Estimating the term…
Abstract
Chimeric transmembrane immunoreceptors (CAR) which include an extracellular domain that includes chlorotoxin or a related toxin, or a variant of chlorotoxin or a related toxin, that binds to human glioma or other human tumor cells, a transmembrane region, a costimulatory domain and an intracellular signaling domain are described.
Claims41
A nucleic acid molecule encoding a chimeric antigen receptor, wherein the chimeric antigen receptor comprises:
- (i) chlorotoxin;
- (ii) a transmembrane domain selected from the group consisting of:
- a CD4 transmembrane domain,
- a CD8 transmembrane domain,
- a CD28 transmembrane domain, and
- a CD3ζ transmembrane domain;
- (iii) a costimulatory domain;
- and
- (iv) CD3ζ signaling domain.
A nucleic acid molecule encoding a chimeric antigen receptor comprising an amino acid sequence selected from the group consisting of:
- an amino acid sequence that is identical to SEQ ID NO:
- 26 or differs from SEQ ID NO:
- 26 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 27 or differs from SEQ ID NO:
- 27 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 28 or differs from SEQ ID NO:
- 28 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 29 or differs from SEQ ID NO:
- 29 no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 30 or differs from SEQ ID NO:
- 30 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 31 or differs from SEQ ID NO:
- 31 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 32 or differs from SEQ ID NO:
- 32 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 33 or differs from SEQ ID NO:
- 33 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 34 or differs from SEQ ID NO:
- 34 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 35 or differs from SEQ ID NO:
- 35 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 36 or differs from SEQ ID NO:
- 36 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 37 or differs from SEQ ID NO:
- 37 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 38 or differs from SEQ ID NO:
- 38 by no more than 5 single amino acid substitutions;
- an amino acid sequence that is identical to SEQ ID NO:
- 39 or differs from SEQ ID NO:
- 39 by no more than 5 single amino acid substitutions;
- and an amino acid sequence that is identical to SEQ ID NO:
- 40 or differs from SEQ ID NO:
- 40 by no more than 5 single amino acid substitutions.
A nucleic acid molecule encoding a chimeric antigen receptor, wherein the chimeric antigen receptor comprises:
- chlorotoxin comprising SEQ ID NO:
- 1;
- a spacer comprising a sequence selected from the group consisting of:
- SEQ ID NOs:
- 2-12;
- a transmembrane domain comprising a sequence selected from the group consisting of SEQ ID NOs:
- 13-20;
- a costimulatory domain comprising a sequence selected from the group consisting of SEQ ID NOs:
- 21-25, and a CD3ζ signaling domain comprising SEQ ID NO:
- 21.
Dependencies are inferred from the claim text.