Alkaline stable immunoglobulin-binding proteins
AssigneeNavigo Proteins GmbH
InventorsPaul Knick, Erik Fiedler, Ulrich Haupts, Maren Meysing
Life of this patent
Estimating the term…
Abstract
The present invention relates to immunoglobulin (Ig) binding proteins comprising one or more Ig binding domains with amino acids selected from the group consisting at least of 1I, 11A, 11E, 11I, 35R, 35I, and 42L. The invention further relates to affinity matrices comprising the Ig binding proteins of the invention. The invention also relates to a use of these Ig binding proteins or affinity matrices for affinity purification of immunoglobulins and to methods of affinity purification using the Ig binding proteins of the invention.
Claims21
An Immunoglobulin (Ig) binding protein comprising one or more Ig binding domains, wherein at least one Ig binding domain comprises SEQ ID NO:
- 1 or SEQ ID NO:
- 2 having 3 or 4 substitutions, wherein the 3 or 4 substitutions are selected from the group consisting of an amino acid substitution to Isoleucine at position 1, an amino acid substitution to Alanine, Glutamic Acid, or Isoleucine at position 11, an amino acid substitution to Arginine or Isoleucine at position 35, and an amino acid substitution to Leucine at position 42, and optionally wherein said at least one Ig binding domain further comprises 1 or 2 additional amino acid sequence modifications, wherein each individual amino acid sequence modification is selected from the group consisting of a single amino acid substitution, a single amino acid deletion, and a single amino acid insertion.
An Immunoglobulin (Ig) binding protein comprising one or more Ig binding domains, wherein at least one Ig binding domain comprises SEQ ID NO:
- 2 having 3 or 4 substitutions, wherein the 3 or 4 substitutions are selected from the group consisting of an amino acid substitution to Isoleucine at position 1, an amino acid substitution to Alanine, Glutamic Acid, or Isoleucine at position 11, an amino acid substitution to Arginine or Isoleucine at position 35, and an amino acid substitution to Leucine at position 42.
An Ig binding protein comprising one or more Ig binding domains, wherein at least one Ig binding domain comprises an amino acid sequence of SEQ ID NO:
- 52, wherein the amino acid at position 5 is selected from H or F, the amino acid at position 7 is selected from K or E, the amino acid at position 8 is selected from D, A, or E, the amino acid at position 25 is selected from D or E, the amino acid at position 28 is selected from S or N, the amino acid at position 42 is selected from L or K, the amino acid at position 44 is selected from I or V, the amino acid at position 46 is selected from G or A, the amino acid at position 49 is selected from K or Q, the amino acid at position 54 is selected from A or S, and the amino acid at position 58 is selected from P or K.
Dependencies are inferred from the claim text.