Insoluble fusion protein comprising antimicrobial peptide and method for producing antimicrobial peptide using same

Abstract

Disclosed is a method of producing an antimicrobial peptide wherein an antimicrobial peptide gene is fused with a green fluorescent protein gene expressed insolubly in E. coli, followed by introduction into E. coli, expression, and removal of the green fluorescent protein to yield the antimicrobial peptide. This method is capable of producing the antimicrobial peptide with high yield in a simple and economical manner and is thus effective at providing native antibiotics that can replace conventional antibiotics in pharmaceutical and feed industries, requiring the development of antibiotics having a new mechanism of action that can eradicate resistant strains due to the proliferation of multiple-drug-resistant microorganisms. Furthermore, the use of an amino acid cleavage process through acid treatment, instead of using conventional cyanogen bromide, is cost-effective for the purification of a target protein from an insoluble protein, can decrease the risk of processing, and enables rapid processing.

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