Back to results
US 11,230,573 B2Granted
Hexon protein hypervariable region gene sequence of adenovirus and its application
AssigneeJiaxing Anyu Biotechnology Co., Ltd.
InventorsPing Chen, Na Li, Xintao Zhong, Tingting Zhang, Nan Li
Life of this patent
Estimating the term…
Abstract
The invention provides a gene sequence which can encode and express adenovirus hexon protein in vitro, and is represented as SEQ ID NO: 3. Also invented a protein which was translated and expressed by the gene sequence according to the invention, and the invention also relates to the use of the protein as an antigen to immunize rabbits to obtain a polyclonal antibody. The antibody mentioned above can detect adenovirus with high sensitivity and specificity.
Claims3
A method for producing an adenovirus hexon protein, comprising:
- using two primers to amplify Hexon-HVR full-length gene sequence to get a nucleotide sequence consisting of SEQ ID NO:
- 3, wherein the two primers are nucleotide sequences consisting of SEQ ID NO:1 and SEQ ID NO:2;
- inserting the nucleotide sequence consisting of SEQ ID NO:
- 3 into a plasmid vector;
- and
- transferring the vector with the nucleotide sequence consisting of SEQ ID NO:
- 3 into Escherichia coli to express said adenovirus hexon protein, wherein the Hexon-HVR full-length gene sequence is the serial number of AC 000008.1 in NCBI, and wherein the hexon protein has the amino acid sequence of SEQ ID NO:
- 4.
5 more elements
Dependencies are inferred from the claim text.