Lateral flow assay housing with integrated sample and buffer solution delivery and measurement

A lateral flow assay device, comprising:

• a capillary pad configured to:
• receive a sample fluid;
• and
• move the sample fluid by capillary action;
• a test line for determining whether the sample fluid comprises a target analyte;
• a sample port configured to:
• receive the sample fluid;
• hold the sample fluid prior to a hole made in a wall of the sample port;
• and
• apply the sample fluid to the capillary pad after the hole is made in the sample port's wall;
• a cap comprising a sealer, the sealer configured to:
• fit inside the sample port after the sample port receives the sample fluid;
• form a compartment for holding a predetermined volume of sample fluid between said sample port's wall and the sealer;
• and
• push any amount of sample fluid in excess of the predetermined volume out of the compartment after the cap is fitted inside the sample port;
• a breaker comprising a tip, the breaker configured to:
• receive a force to press the tip against the wall of the sample port;
• and
• in response to receiving the force, make a hole in the wall of the sample port with the tip causing the sample fluid held inside the compartment to be applied to the capillary pad.