Back to results
US 11,229,896 B2Granted
Affinity chromatography media and chromatography devices
AssigneesW.R. Grace & Co.-Conn., Repligen Corporation
InventorsFeng Gu, Surya Kiran Chodavarapu, James Bogdanor, Dennis McCreary, James Neil Pryor, James Rusche, James Peyser, Thomas Pauly
Life of this patent
Estimating the term…
Abstract
Chromatography media and devices containing chromatography media are disclosed. Methods of making chromatography devices and methods of using chromatography devices containing the chromatography media are also disclosed.
Claims29
Chromatography media comprising:
- porous inorganic particles having a median pore diameter of from 600 Å to 1600 Å;
- a pore size distribution relative span of at least 1.0 up to 2.0;
- a pore size distribution such that at least 40% of a total pore volume of said porous inorganic particles comprises pores in a diameter range of from 600 Å to 1600 Å;
- and a functionalized surface comprising an average of from greater than 1 to 2.5 linker molecules per square nanometer of surface area of said porous inorganic particles.
1 more element
Chromatography media comprising:
- porous silica particles having a median pore diameter of from 600 Å to 1600 Å;
- a pore size distribution relative span of at least 1.0 up to 2.0;
- at least 40% of the total pore volume is in pores having a diameter between 600 Å to 1600 Å;
- and a functionalized surface comprising a protein covalently bonded to surface portions of said porous silica particles, said protein having a molecular weight ranging from 10,000 to 100,000 D, wherein said protein is covalently bonded to surface portions of said porous silica particles via an average of greater than 1.3 to 2.5 linker molecules per square nanometer of surface area of said porous silica particles.
1 more element
Chromatography media comprising:
- porous silica particles having an average BET surface area of from about 20 m2/g to 100 m2/g;
- an average pore volume of at least 1 ml/g;
- a pore size distribution relative span of at least 1.0 up to 2.0;
- at least 40% of the total pore volume is in pores having a diameter between 600 Å to 1600 Å;
- a functionalized surface comprising an average of from greater than 1.3 to 2.5 linker molecules per square nanometer of surface area of said porous inorganic particles;
- and a protein covalently bonded to at least some of said linker molecules, said protein having a molecular weight ranging from 10,000 to 100,000 D, wherein said chromatography media, when used in an affinity column, (i) has a non-specific protein binding level of less than 20.0 ng/ml, and (ii) has a dynamic binding capacity of at least 30 mg/ml.
3 more elements
Chromatography media comprising:
- porous inorganic particles having a median pore diameter of from 600 Å to 1600 Å;
- a pore size distribution relative span of at least 1.0 up to 2.0;
- a pore size distribution such that (i) at least 20% of a total pore volume of said porous inorganic particles comprises pores in a diameter range of from 1000 Å to 1600 Å, and (ii) at least 15% of the total pore volume of said porous inorganic particles comprises pores in a diameter range from 1200 Å to 1500 Å;
- and a functionalized surface.
1 more element
Dependencies are inferred from the claim text.