System and method for emulsion breaking
AssigneeLife Technologies Corporation
InventorsBrian Reed, James A. Ball
Life of this patent
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Abstract
A method of recovering a bead support from an emulsion includes supplying an aqueous surfactant solution into a centrifuge tube; supplying a hydrophobic liquid over the surfactant solution in the centrifuge tube, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the hydrophobic liquid is not greater than 0.5; and applying an emulsion over the hydrophobic liquid while centrifuging, the emulsion comprising a dispersed aqueous phase including the bead support, the emulsion breaking and material of the dispersed phase preferentially partitioning to the surfactant solution.
Claims7
A method of recovering a bead support from an emulsion, the method comprising:
- supplying an aqueous surfactant solution into a centrifuge tube;
- the surfactant solution including an anionic surfactant in a range of 5.0% to 15.0% and including a non-ionic surfactant in a range of 0.05% to 8.0%, wherein a ratio of the volume of the aqueous surfactant solution to the volume of the centrifuge tube is not greater than 0.5 and at least 0.01;
- and
- preparing an emulsion comprising a sample of template bead supports in a dispersed aqueous phase and a hydrophobic liquid in a continuous phase;
- applying the emulsion into the centrifuge tube while centrifuging;
- recovering the sample of template bead supports from the surfactant solution;
- washing the recovered sample of template bead supports;
- and
- applying the washed sample of template bead supports to an array of wells, wherein the array of wells forms a part of a genetic sequencing system for performing genetic testing on the sample.
Dependencies are inferred from the claim text.