System and method for harvesting autologous adipose tissue

A method of capturing and separating adipose tissue components comprising:

• (a) providing a triple-chambered container;
• the triple chambered container comprising:
• a main container body defining therein a first chamber and having an opening at its distal end
• a main barrel formed and movable longitudinally within the first chamber, the main barrel defining therein a second chamber for receiving fluids, the main barrel further having a first apertured stopper at its distal end;
• a second barrel formed within the main barrel, the second barrel defining a third chamber for receiving fluids, the second barrel being movable longitudinally within and with respect to the main barrel, the second barrel having a distal end with a second apertured stopper;
• a shaft adapted to fit within the second barrel, the shaft being movable longitudinally within and with respect to the second barrel, the shaft having a distal end which is engageable and disengageable with the second apertured stopper in the second barrel;
• a device for controlling a movement of the distal end of the shaft with the aperture of the second barrel;
• and,
• wherein the second chamber and the third chamber may be selectively moved to receive and discharge fluids with respect to one another;
• wherein the method comprises:
• (b) providing a volume of adipose tissue to the first chamber;
• (c) centrifuging the container so that the adipose tissue is separated into four components;
• (d) the four components being, in order proximally to distally:
• oils, fat, blood components, and adipose-derived stem cells;
• (e) moving the oils into the third chamber by moving the shaft to disengage it from the aperture in the second apertured stopper and moving the main barrel in the direction of the distal end of the first chamber until the oils are contained in the second barrel;
• (f) closing the second apertured stopper by engaging the shaft with the aperture in the second apertured stopper such that the oils are constrained to remain within the third chamber;
• (g) moving the fat into the second chamber by moving the second barrel in a proximal direction, thus opening the first apertured stopper, then advancing the main barrel in a distal direction so that the fat enters into second chamber;
• (h) closing the first apertured stopper with the second barrel such that the fat is constrained to remain within the second chamber;
• (i) moving the blood components into the third chamber by moving the shaft to disengage it from the aperture in the second apertured stopper and moving the main barrel in the direction of the distal end of the first chamber until the blood components are contained in the second barrel with the oils;
• (j) closing the second apertured stopper such that the blood components and the oils are constrained to remain within the third chamber;
• and,
• (k) moving the adipose-derived stem cells into the second chamber by moving the second barrel in a proximal direction, thus opening the first apertured stopper, then advancing the main barrel in a distal direction so that the adipose-derived stem cells enter into second chamber with the fat.