In vivo detection of a xenon-binding cage molecule
AssigneeLakehead University
InventorsMitchell Albert, Francis Hane
Life of this patent
Estimating the term…
Abstract
Xenon based biosensors have the potential to detect and localize biomarkers associated with a wide variety of diseases. The development and nuclear magnetic resonance (NMR) characterization of cage molecules which encapsulate hyperpolarized xenon is imperative for the development of these xenon biosensors. We acquired 129Xe NMR spectra, and magnetic resonance images and a HyperCEST saturation map of cucurbituril (CB6) in whole bovine blood. We observed a mean HyperCEST depletion of 84% (n=5) at a concentration of 5 mM and 74% at 2.5 mM. Additionally, we collected these data using a pulsed HyperCEST saturation pre-pulse train with a SAR of 0.025 W/kg which will minimize any potential RF heating in animal or human tissue.
Claims14
An in vivo method of magnetic resonance imaging comprising:
- injecting a solution comprising tagged supramolecular xenon cages into a subject;
- while ventilating said subject with a mixture of oxygen gas and hyperpolarized xenon gas such that xenon in the tagged supramolecular xenon cages exchange with the hyperpolarized xenon gas, thereby forming tagged supramolecular hyperpolarized xenon cages, said tagged supramolecular hyperpolarized xenon cages having a chemical shift frequency;
- applying a first saturation pre-pulse comprising of sixteen 6 ms 3-lobe sinc pulses with 3 ms pulse intervals at the chemical shift frequency, thereby depolarizing xenon in the tagged supramolecular hyperpolarized xenon cages;
- generating an on-resonance xenon magnetic resonance image of the subject at the chemical shift frequency;
- applying a second saturation pre-pulse comprising of sixteen 6 ms 3-lobe sinc pulses with 3 ms pulse intervals at an off-resonance frequency;
- generating an off-resonance xenon magnetic resonance image of the subject at the off-resonance frequency;
- and
- subtracting the off-resonance xenon magnetic resonance image from the on-resonance xenon magnetic resonance image, thereby imaging the location of the tagged supramolecular xenon cages within the subject.
An in vivo method of magnetic resonance imaging comprising:
- injecting a solution comprising tagged cucurbituril xenon cages into a subject, said tagged cucurbituril xenon cages having a chemical shift frequency;
- while ventilating said subject with a mixture of oxygen gas and hyperpolarized xenon gas such that the xenon in the tagged cucurbituril xenon cages exchanges with the hyperpolarized xenon gas, thereby forming tagged cucurbituril hyperpolarized xenon cages, said tagged cucurbituril hyperpolarized xenon cages having a chemical shift frequency;
- applying a first saturation pre-pulse comprising of sixteen 6 ms 3-lobe sinc pulses with 3 ms pulse intervals at the chemical shift frequency, thereby depolarizing xenon in the tagged cucurbituril xenon cages;
- generating an on-resonance xenon magnetic resonance image of the subject at the chemical shift frequency;
- applying a second saturation pre-pulse comprising of sixteen 6 ms 3-lobe sinc pulses with 3 ms pulse intervals at an off-resonance frequency;
- generating an off-resonance xenon magnetic resonance image of the subject at the off-resonance frequency;
- and
- subtracting the off-resonance xenon magnetic resonance image from the on-resonance xenon magnetic resonance image, thereby imaging the location of the tagged cucurbituril xenon cages within the subject.
Dependencies are inferred from the claim text.