Recombinant non-pathogenic Marek's Disease virus constructs encoding multiple heterologous antigens

A recombinant herpesvirus of turkeys (rHVT) that comprises at least three heterologous nucleotide sequences;

• wherein the first heterologous nucleotide sequence is comprised by a first heterologous nucleic acid or a second heterologous nucleic acid, the second heterologous nucleotide sequence is comprised by the first heterologous nucleic acid or the second heterologous nucleic acid, and the third heterologous nucleotide sequence is comprised by the first heterologous nucleic acid or the second heterologous nucleic acid;
• wherein the first heterologous nucleic acid is located in a first nonessential site in the rHVT genome, and the second heterologous nucleic acid is located in a second nonessential site in the rHVT genome;
• wherein the first nonessential site and the second nonessential site are either the same or different;
• wherein the first heterologous nucleotide sequence comprises a coding sequence for an Infectious Bursal Disease Virus viral protein 2 (IBDV VP2), the second heterologous nucleotide sequence comprises a coding sequence for an Infectious Laryngotracheitis Virus glycoprotein D (ILTV gD) and a coding sequence for an Infectious Laryngotracheitis Virus glycoprotein I (ILTV gI), and the third heterologous nucleotide sequence comprises a coding sequence for a Newcastle Disease Virus fusion protein (NDV F);
• wherein when the first nonessential site and the second nonessential site are two different sites, the two different sites are the US2 site and the UL54.5 site;
• and
• wherein when the first nonessential site and the second nonessential site are the same site, the site is selected from the group consisting of the US2 site and the UL54.5 site.