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US 11,222,423 B2Granted
Evaluation device, observation device, and program for identifying cell differentiation
AssigneesNikon Corporation, National Institutes of Biomedical Innovation, Health and Nutrition
InventorsHiroaki Kii, Yasujiro Kiyota, Takayuki Uozumi, Miho Furue, Mika Suga
Life of this patent
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Abstract
An evaluation device includes a state determination unit which is configured to determine a state of cells which are an observation target under non-standard conditions on the basis of information acquired from an image of cells under standard conditions.
Claims10
An evaluation device comprising:
- a processor;
- a memory encoded with instructions executed by the processor, the instructions causing the processor to perform operations steps comprising:
- extracting an image of neural cells from images captured at more than one time point, wherein the extracted image comprises a differentiated state of the neural cells, and wherein the extracted image is identified using observation targets;
- selecting more than one marker, wherein each of the more than one marker is suitable for evaluating differentiated states of the neural cells and immunostaining to evaluate differentiated states of the neural cells, and each of the more than one marker representing a differentiation process of the neural cells corresponding to at least one of the observation targets;
- storing, as a control experimental result, the extracted image of neural cells including a marker change pattern of the selected marker, wherein the extracted image is extracted after cultivation of a neural cell of a cell type that corresponds to the observation targets under a standard condition using a combination of the more than one selected markers, wherein the standard condition comprises a condition where a substance which potentially influences the neural cells in a process of differentiation induction has not been added to the neural cells;
- comparing an experimental result based on the extracted image of neural cells with the control experimental result to determine a differentiated state of the neural cells which are observation targets after a cell cultivation under a non-standard condition using the combination of more than one selected markers selected for the neural cells, wherein the non-standard condition comprises a condition where the substance has been added to the neural cell;
- and wherein the differentiated state of the neural cells is determined based on the marker change pattern in expression state of the facilitated marker and the suppressed marker.
4 more elements
A non-transitory medium storing a program which, when executed on a computer, causes the computer to perform the following:
- an image acquisition step of acquiring a captured image of cells;
- an observation result acquisition step comprising acquiring an observation result of cells under a standard condition;
- and
- a cell image extraction step comprising extracting an image of neural cells from images captured at more than one time point, wherein the extracted image comprises a differentiated state of the neural cells, and wherein the extracted image is identified using observation targets;
- a marker selection step comprising selecting more than one marker, wherein each of the more than one marker is suitable for evaluating differentiated states of the neural cells and immunostaining to evaluate differentiated states of the neural cells, and each of the more than one marker representing a differentiation process of the neural cells corresponding to at least one of the observation targets;
- and
- storing, as a control experimental result, the extracted image of neural cells including a marker change pattern of the selected marker, wherein the cell image extraction step occurs after cell cultivation of a neural cell whose cell type is the same as the observation targets under a standard condition using a combination of more than one of the markers selected by the marker selection step, wherein the standard condition comprises a condition where a substance which potentially influences the neural cells in a process of differentiation induction has not been added to the neural cells;
- a state determination step of comparing an experimental result based on the extracted image of neural cells extracted with the control experimental result stored in the storage step to determine a differentiation state of the neural cells which are the observation targets after a cell cultivation under a non-standard condition using a combination of a plurality of the markers selected for the neural cells which are the observation targets in the marker selection step, wherein the non-standard condition comprises a condition where the substance has been added to the neural cell;
- wherein the combination of the more than one marker selected in the marker selection step includes a facilitated marker of which expression is facilitated with progress of differentiation induction of the neural cells and a suppressed marker of which expression is suppressed with the progress of differentiation induction of the neural cells,
- wherein the state determination step determines the differentiation state of the neural cells based on change of an expression states of the facilitated marker and the suppressed marker, and
- wherein the information on neural cells under the non-standard conditions is information on neural cells in process of differentiation induction under a condition where a substance which potentially influences the neural cells in process of induction differentiation has been added, and the information on neural cells under the standard conditions is information under a condition where the substance has not been added to the neural cells in process of differentiation.
8 more elements
Dependencies are inferred from the claim text.