Protein-matrix microlens array diffraction device and method for preparing the same
AssigneeTsinghua University
InventorsLan Jiang, Jianfeng Yan, Jiachen Yu
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Abstract
Provided are a protein-matrix microlens array diffraction device and a preparation method thereof. The protein-matrix microlens array diffraction device includes a matrix of a protein crystal. A largest side of the protein crystal has a length of 100 to 500 μm, a surface of the protein crystal where the largest side is located is processed to have an array of microlens-like protrusions, a distance p between two adjacent microlens-like protrusions of the array of microlens-like protrusions is in a range of 10 to 100 μm, a diameter d of the microlens-like protrusion is in a range of 2 to 10 μm, and a height h of the microlens-like protrusion is in a range of 0.05 to 2 μm.
Claims7
A protein-matrix microlens array diffraction device, characterized in that the protein-matrix microlens array diffraction device comprises a matrix of a protein crystal,
- a largest side of the protein crystal has a length of 100 to 500 μm,
- a surface of the protein crystal where the largest side is located is processed to have an array of microlens-like protrusions,
- a distance p between two adjacent microlens-like protrusions of the array of microlens-like protrusions is in a range of 10 to 100 μm,
- a diameter d of the microlens-like protrusion is in a range of 2 to 10 μm, and
- a height h of the microlens-like protrusion is in a range of 0.05 to 2 μm.
A method for preparing a protein-matrix microlens array diffraction device, comprising the following steps:
- (1) preparing a protein crystal, comprising:
- (1-1) mixing a protein solid with a solution having a concentration of a salt, comprising sodium chloride and sodium acetate, of 2% to 5% by mass to obtain a protein solution, wherein a mass fraction of the protein in the protein solution is 1 to 3 times a mass fraction of a saturated solution, and the protein solution is adjusted to have a pH of 4 to 6;
- and
- (1-2) placing the protein solution obtained from step (1-1) in a container and leaving the protein solution to stand at a room temperature for a period of 6 to 24 h, wherein during the period water of the protein solution is naturally evaporated, thereby obtaining the protein crystal,
- and
- (2) emitting a femtosecond laser on a surface of the protein crystal obtained in step (1), wherein the femtosecond laser has a pulse duration of 35 to 120 fs, a single pulse energy of the femtosecond laser is half of a protein ablation threshold, and the femtosecond laser has a repetition frequency of 1 to 1000 Hz.
Dependencies are inferred from the claim text.