US 11,220,701 B2 · Method for enzymatic preparation of R-3 aminobutyric acid

Abstract

An R-3-aminobutyric acid preparation method with high efficiency and high stereoselectivity. The method comprises using aspartase with stereoisomerization catalytic activity derived from Escherichia coli to efficiently convert butenoic acid into R-3-aminobutyric acid. After only 24 h of reaction, the conversion rate is as high as ≥98%, and the ee value is ≥99.9%. The conversion efficiency is greatly improved, the reaction time is shortened, and the production costs are reduced. The method features a high yield, a high conversion rate, low costs, a short production cycle, a simple process, ease of enlargement, suitability for mass production and the like.

Claims6

Dependent claims

A method for producing an R-3-aminobutyric acid comprising the steps of:

(1) using butenoic acid as a substrate and carrying out a stereoisomeric catalytic reaction shown in equation I to form the R-3-aminobutyric acid under the catalysis of an aspartase in a reaction system;
(b) optionally, isolating the R-3-aminobutyric acid from the reaction system after the reaction of the step (1);
wherein, the aspartase is obtained from E. coli, and
the aspartase is a mutant having 90% sequence identity to the amino acid sequence of SEQ ID NO:
5 which has two to four amino acid mutations in the amino acid sequence corresponding to the wild type aspartase shown in SEQ ID NO:
5, wherein the amino acid mutation is selected from the group consisting of threonine (T) at position 204, methionine (M) at position 338, Lysine (K) at position 341, asparagine (N) at position 343, or a combination thereof.

3 more elements

Dependencies are inferred from the claim text.

Method for enzymatic preparation of R-3 aminobutyric acid

Life of this patent

Method for enzymatic preparation of R-3 aminobutyric acid

Life of this patent

Abstract

Claims6