US 11,220,691 B2Granted

Methods for high taurine production in unicellular organisms

AssigneePlant Sensory Systems, LLC

InventorsFrank J. Turano, Michelle B. Price

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Abstract

The present invention describes an approach to produce or increase hypotaurine or taurine production in unicellular organisms. More particularly, the invention relates to genetic modification of unicellular organisms that include bacteria, algal, microalgal, diatoms, yeast, or fungi. The invention relates to methods to increase taurine levels in the cells by binding taurine or decreasing taurine degradation. The invention can be used in organisms that contain native or heterologous (transgenic) taurine biosynthetic pathways or cells that have taurine by enrichment. The invention also relates to methods to increase taurine levels in the cells and to use the said cells or extracts or purifications from the cells that contain the invention to produce plant growth enhancers, food, animal feed, aquafeed, food or drink supplements, animal-feed supplements, dietary supplements, health supplements or taurine.

Claims5

Dependent claims

A cell comprising:

(a) a genetic deletion or disruption of one or more of the genes selected from the group consisting of the TauD gene, the SsuD gene, SsuE gene and the Cbl gene and
(b) an expression cassette that comprises a promoter operably linked to a polynucleotide which encodes a cysteine synthetase/PLP decarboxylase (CS/PLP-DC) protein having the amino acid sequence set forth in SEQ ID NO:12,
wherein the cell is an Escherichia coli cell or a Corynebacterium glutamicum cell,
wherein the TauD gene encodes a protein having the amino acid sequence set forth in SEQ ID NO:21, the SsuD gene encodes a protein having an amino acid sequence set forth in SEQ ID NO:23 or 27, the SsuE gene encodes a protein having an amino acid sequence set forth in SEQ ID NO:25 or 29 and the Cbl gene encodes a protein having an amino acid sequence set forth in SEQ ID NO:37 or 39,
wherein the cell has no expression of the gene product of one or more of the TauD gene, the SsuD gene, the SsuE gene and the Cbl gene,
wherein the expression cassette is expressed in the cell and
wherein the cell produces taurine.

4 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/919,623Filed 2 Jul 2020 · division
Published: US 2020/0332301 A122 Oct 2020
Granted: 11 Jan 2022
Term ends: Not estimatedThe source records don’t support an estimate.

Classification17 codes

C12N 15/52
DNA or RNA fragments; Modified forms thereof ; Non-coding nucleic acids having a biological activity: genes encoding for enzymes or proenzymes
A23K 10/12
Obtained by microbiological or biochemical processes: by fermentation of natural products, e.g. of vegetable material, animal waste material or…
A23K 50/80
Feeding-stuffs specially adapted for particular animals: for aquatic animals, e.g. fish, crustaceans or molluscs
C12N 9/0069
Oxidoreductases: acting on single donors with incorporation of molecular oxygen, i.e. oxygenases

Family

PCT/US2016/028958Parent of this division · filed 22 Apr 2016
US 2020/0332301 A1Pre-grant publication · published 22 Oct 2020
Application16/092,363Parent of this division
US 2019/0085339 A1Published application
US 11,220,691 B2This patent

From the US filing history. Foreign family members aren’t listed.