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US 11,220,670 B2Granted
Remnant tumor infiltrating lymphocytes and methods of preparing and using the same
AssigneeIovance Biotherapeutics, Inc.
InventorsMichelle R. Simpson-Abelson, Christopher Mosychuk, Michael T. Lotze
Life of this patent
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Abstract
In some embodiments, methods of delivering a therapeutically effective amount of an expanded number of tumor infiltrating lymphocytes obtained from tumor remnants to a patient in need thereof, for the treatment of a cancer, are disclosed.
Claims18
A method of treating a cancer in a patient in need of such treatment, the method comprising:
- obtaining a therapeutically effective amount of remnant tumor infiltrating lymphocytes (rTILs), by enzymatically digesting tumor remnants separated from emergent tumor infiltrating lymphocytes (eTILs) using a digest mixture comprising a deoxyribonuclease, a collagenase, a hyaluronidase, or a combination thereof, and expanding the rTILs with a second cell culture medium comprising cell culture media, irradiated feeder cells, OKT-3 antibody, and interleukin 2 (IL-2), wherein the tumor remnants and the eTILs are derived from tumor tissue that was obtained from the patient, fragmented and treated with a first cell culture medium comprising IL-2,
- and administering the therapeutically effective amount of rTILs to the patient.
Dependencies are inferred from the claim text.