Two-photon fluorescent compounds for specific lipid droplet imaging in live cells and deep tissues at ultralow concentration
AssigneeThe Hong Kong University of Science and Technology
InventorsBenzhong Tang, Guangle Niu
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Abstract
Fluorescent compounds that have aggregation-induced emission (AIE) characteristics. The compounds can be utilized as lipid droplet (LD)-specific bio-probes in cell imaging, with high photostability and brightness. For example, the compounds can be used for specific two-photon LDs staining in live cells and deep-tissues at ultralow concentrations. The compounds exhibit a large Stokes shift (>110 nm), high solid fluorescence quantum yields (up to 0.30), a good two-photon absorption cross-section (45-100 GM at 860 nm), high biocompatibility, and good photostability.
Claims20
A fluorescent compound exhibiting aggregation induced emission properties, the compound having the following backbone structural formula:
- wherein R1, R2, and R3 are independently selected from the group consisting of CnH2n+1, C6H5, C10H7, CnH2nCOOH, CnH2nNCS, CnH2nN3, CnH2nNH2, CnH2nCl, CnH2nBr, CnH2nI and
- wherein R′ is selected from the group consisting of CnH2nNCS, CnH2nN3, CnH2nNH2, CnH2nCl, CnH2nBr, CnH2nI, and
- wherein R4, R5, and R6 are independently selected from the group consisting of H, CH3, F, Cl, Br, I, CN, CF3, NO2, Ph, Py, CH═CHPh, and C≡CPh;
- and
- wherein n is an integer ranging from 1 to 10.
A fluorescent compound exhibiting aggregation induced emission properties, the compound comprising one or more compounds selected from the group consisting of:
A method of cellular imaging, comprising:
- contacting a target cell with a compound having the following backbone structural formula
- wherein R1, R2, and R3 are independently selected from the group consisting of CnH2n+1, C6H5, C10H7, CnH2nCOOH, CnH2nNCS, CnH2nN3, CnH2nNH2, CnH2nCl, CnH2nBr, CnH2nI and
- wherein R′ is selected from the group consisting of CnH2nNCS, CnH2nN3, CnH2nNH2, CnH2nCl, CnH2nBr, CnH2nI, and
- wherein R4, R5, and R6 are independently selected from the group consisting of H, CH3, F, Cl, Br, I, CN, CF3, NO2, Ph, Py, CH═CHPh, and C≡CPh;
- and
- wherein n is an integer ranging from 1 to 10;
- and
- identifying a target of interest in the target cell using an imaging method.
Dependencies are inferred from the claim text.