Recombinant bagworm silk
AssigneeNational Agriculture and Food Research Organization
InventorsNaoyuki Yonemura, Tetsuya Iizuka, Kenichi Nakajima, Takuya Tsubota, Takao Suzuki, Hideki Sezutsu, Tsunenori Kameda, Taiyo Yoshioka
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Abstract
A method of producing a transgenic silkworm that spins bagworm silks and producing a large quantity of bagworm silks by transgenic technology is developed and provided. A gene encoding a modified bagworm Fib H and a transgenic silkworm in which the gene is introduced, wherein the gene is obtained by cloning a gene fragment encoding a bagworm Fib H-like polypeptide comprising a partial amino acid sequence of bagworm Fib H, and fusing the gene fragment to a gene fragment encoding silkworm-derived Fib H, are provided.
Claims10
A gene encoding a modified bagworm fibroin H chain, wherein the modified bagworm fibroin H chain is a chimeric fibroin H chain comprising a bagworm fibroin H chain fused to a portion of a silkworm fibroin H chain, wherein the modified bagworm fibroin H chain comprises (a) or (b):
- (a) an amino acid sequence comprising positions 1 to 153 of SEQ ID NO:
- 9,
- one or a plurality of the amino acid sequences set forth in SEQ ID NOs:
- 5 and/or 7, and
- an amino acid sequence comprising positions 466 to 524 of SEQ ID NO:
- 9,
- (b) an amino acid sequence comprising positions 1 to 153 of SEQ ID NO:
- 9,
- one or a plurality of an amino acid sequence having an amino acid identity of 90% or more to full length SEQ ID NOs:
- 5 and/or 7, and
- an amino acid sequence comprising positions 466 to 524 of SEQ ID NO:
- 9.
A method of producing a transgenic silkworm that spins a modified bagworm silk, comprising:
- crossing a transgenic silkworm having a first expression unit comprising a promoter for expression in the posterior silk gland derived from a silk spinning insect and a gene encoding a transcriptional regulator placed under the downstream control of the promoter and a transgenic silkworm having a second expression unit comprising a promoter targeted by the transcriptional regulator and the modified bagworm fibroin H chain gene placed under the downstream control of the targeted promoter;
- and
- selecting a transgenic silkworm having the first and second expression units from progenies after the cross.
Dependencies are inferred from the claim text.