Articles and methods directed to personalized therapy of cancer

A method of treating lymphoma in a subject comprising:

• obtaining B cells from a biopsy from the subject;
• extracting RNA from the cells;
• synthesizing cDNA from the extracted RNA;
• amplifying the variable region genes of heavy and light Ig chains;
• sequencing the variable region genes;
• and identifying a unique B cell receptor expressed in the lymphoma cells of the subject based on the sequencing of the variable region genes from the B cells;
• expressing the B cell receptor in membrane bound form and expressing a library of putative B cell receptor peptide ligands;
• detecting binding of said unique B cell receptor to a putative unique B cell receptor peptide ligand, thereby identifying a B cell receptor peptide ligand that binds the unique B cell receptor expressed in the lymphoma cells of the subject;
• concomitantly administering to the subject chimeric antigen receptor (CAR)-expressing T-cells, wherein the CAR comprises an antigen binding domain comprising the peptide ligand that specifically binds the unique B cell receptor expressed in the lymphoma cells of the subject;
• and a vaccine comprising a polypeptide comprising the unique B cell receptor expressed in the lymphoma cells of the subject or a fragment thereof comprising at least one variable domain sequence or a nucleic acid expressing the unique B cell receptor expressed in the lymphoma cell of the subject or a fragment thereof comprising at least one variable domain sequence.