Recombinant microorganism having heterologous genes introduced thereto and method for producing useful material from formic acid and carbon dioxide using same microorganism

A recombinant microorganism having improved assimilation from formic acid and carbon dioxide, obtained by introducing a gene encoding an enzyme involved in a formic acid assimilation pathway,

• wherein the microorganism is Escherichia coli capable of synthesizing a C3 or larger carbon compound under the formic acid assimilation pathway combined with a cyclic pathway for fixing carbon dioxide,
• wherein the gene encoding an enzyme involved in a formic acid assimilation pathway comprises:
• the gene encoding formate-tetrahydrofolate ligase comprising the nucleic acid molecule of SEQ ID NO:
• 7;
• the gene encoding methenyl tetrahydrofolate cyclohydrolase comprising the nucleic acid molecule of SEQ ID NO:
• 8;
• and
• the gene encoding methylene-tetrahydrofolate dehydrogenase comprising the nucleic acid of SEQ ID NO:
• 9 or SEQ ID NO:
• 19;
• and
• wherein the gene encoding an enzyme involved in the cyclic pathway for fixing carbon dioxide comprises one or more genes selected from:
• the gene encoding a serine-glyoxylate transaminase comprising the nucleic acid of SEQ ID NO:
• 6, SEQ ID NO:
• 13, SEQ ID NO:
• 16, or SEQ ID NO:
• 21;
• the gene encoding a malyl-CoA lyase comprising the nucleic acid of SEQ ID NO:
• 5, SEQ ID NO:
• 14, or SEQ ID NO:
• 15;
• and
• the gene encoding a malate-CoA ligase comprising the nucleic acid of SEQ ID NO:
• 3, SEQ ID NO:
• 4, SEQ ID NO:
• 11, or SEQ ID NO:
• 12.