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AssigneePuratos N.V.
InventorsGeertrui Bosmans, Ellen Fierens, Kristof Brijs, Jan Delcour, Fabienne Verte, Jacques Georis, Valérie Dorgeo, Filip Arnaut
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The present invention concerns a method for selectively activating a thermostable hydrolase at a temperature above T1. The present invention provides compositions comprising a thermostable hydrolase and a temperature sensitive inhibitor, wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T1, but which dissociates at a temperature of about T1. The present invention also relates to uses of said compositions, and a method for preparing said compositions.
A composition comprising a thermostable hydrolase and a temperature sensitive inhibitor, wherein the hydrolase is a serine protease, and said inhibitor is of vegetable origin or a recombinant, semi-synthetic, or synthetic variant thereof, and wherein said thermostable hydrolase and said temperature sensitive inhibitor form a hydrolase-inhibitor complex at a temperature below T1, wherein said inhibitor inhibits the activity of said hydrolase in said complex at a temperature below T1 and dissociates from said complex at a temperature of about T1 and T1 is the temperature at which the hydrolase-inhibitor complex dissociates and is at least 60° C.
Dependencies are inferred from the claim text.