Method for biocatalytic production of terpene compounds
OwnerMichel Schalk, Letizia Rocci +1inventors
InventorsMichel Schalk, Letizia Rocci, Daniel Solis Escalante
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Abstract
Provided herein are biocatalytic methods of producing terpene compounds by applying a novel type of phosphatase enzyme. The method allows the fully biochemical synthesis of terpene compounds, like for example copalol and labdendiol, and derivatives thereof, which serve as valuable intermediates for the production of perfumery ingredients, such as, for example, ambrox. Also provided are novel fully biochemical multistep processes for the production of such compounds as well as novel phosphatase enzymes and mutants and variants derived therefrom.
Claims17
A biocatalytic method of producing a terpene alcohol compound, of the general formula 1
- wherein
- R represents H or a cyclic or non-cyclic, linear or branched, saturated or unsaturated, optionally substituted hydrocarbyl residue,
- the method comprising the steps of:
- (1) contacting the corresponding terpenyl diphosphate precursor of said terpene compound of formula (1) with a polypeptide having terpenyl-diphosphate phosphatase activity to form said terpene alcohol;
- and
- (2) optionally isolating the terpene alcohol of step (1)
- wherein said polypeptide having terpenyl-diphosphate phosphatase activity is selected from a diphosphate removing enzyme member of the protein tyrosine phosphatase family.
A biocatalytic method of producing a bicyclic diterpene alcohol compound,
- the method comprising the steps of:
- a) contacting the corresponding bicyclic diterpenyl diphosphate precursor of said bicyclic diterpene compound with a polypeptide having terpenyl-diphosphate phosphatase activity to form said bicyclic diterpene alcohol;
- and
- b) optionally isolating the bicyclic diterpene alcohol of step (a).
Dependencies are inferred from the claim text.