Reagent for extracting and amplifying nucleic acid

A method for extracting and amplifying a nucleic acid of a nucleic acid extraction target from a sample containing the nucleic acid extraction target, comprising (i), (ii) and (iii), wherein the nucleic acid extraction target is selected from microorganisms, animal cells, plant cells, and extracellular vesicles:

• (i) extracting the nucleic acid, which involves contacting a sample containing a nucleic acid extraction target with a nucleic acid extraction reagent containing at least a surfactant with a steroid skeleton, thereby obtaining a nucleic acid extract;
• (ii) contacting the nucleic acid extract obtained in (i) with γ-cyclodextrin having a C1-4 hydroxyalkyl group, wherein said γ-cyclodextrin having a C1-4 hydroxyalkyl group is 2-hydroxypropyl-γ-cyclodextrin;
• and
• (iii) amplifying the nucleic acid, which involves contacting the solution obtained in (ii) with a nucleic acid amplification reagent.

A method for extracting and amplifying a nucleic acid of a nucleic acid extraction target from a sample containing the nucleic acid extraction target, comprising (i) and (ii), wherein

• the nucleic acid extraction target is selected from microorganisms, animal cells, plant cells, and extracellular vesicles:
• (i) extracting the nucleic acid, which involves contacting a sample containing the nucleic acid extraction target with a nucleic acid extraction reagent containing at least a surfactant with a steroid skeleton, thereby obtaining a nucleic acid extract;
• and
• (ii) amplifying the nucleic acid, which involves contacting the nucleic acid extract obtained in (i) with a nucleic acid amplification reagent containing γ-cyclodextrin having a C1-4 hydroxyalkyl group, wherein said γ-cyclodextrin having a C1-4 hydroxyalkyl group is 2-hydroxypropyl-γ-cyclodextrin.