Branched receptor binding multi-subunit protein complexes for use in bacterial delivery vehicles
AssigneeEligo Bioscience
InventorJesus Fernandez Rodriguez
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Abstract
The present disclosure relates generally to bacterial delivery vehicles for use in efficient transfer of a desired payload into a target bacterial cell. More specifically, the present disclosure relates to bacterial delivery vehicles with desired host ranges based on the presence of a chimeric receptor binding protein (RBP) composed of a fusion between the N-terminal region of a RBP derived from a lambda-like bacteriophage and the C-terminal region of a different RBP, and/or the presence of an engineered branched receptor binding multi-subunit polypeptides (“branched-RBP”).
Claims27
An engineered branched receptor binding multi-subunit protein complex (branched-RBP) comprising two or more associated bacteriophage derived receptor binding proteins (RBP):
- wherein the two or more associated bacteriophage derived RBPs comprise an interaction domain (ID) that mediates the association of the RBP's with one another to form the branched-RBP, and
- wherein at least one of the two or more associated bacteriophage derived RBPs is a chimeric RBP comprising a fusion between the N-terminal domain of a RBP from a lambda or lambda-like bacteriophage and the C-terminal domain of a different RBP and wherein the ID is inserted between the N-terminal domain and the C-terminal domain of the chimeric RBP.
Dependencies are inferred from the claim text.