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US 11,206,843 B1Granted
Milk product compositions
AssigneeBIOMILQ, Inc.
InventorLeila Strickland
Life of this patent
Estimating the term…
Abstract
Disclosed herein are milk compositions that comprise protein, lipid, and oligosaccharide components at concentrations that mimic and/or are substantially similar to human breast milk as produced by a lactating female. The milk compositions include one or more milk components produced in vitro and/or ex vivo from cultured mammary cells.
Claims13
A method of producing an isolated milk product comprising secretory IgA (sIgA) from cultured mammary cells and plasma cells, the method comprising:
- a. culturing a live cell construct in a bioreactor under conditions which produce the milk product, said cell construct comprising:
- i. a three-dimensional scaffold having an exterior surface, an interior surface defining an interior cavity, and a plurality of pores extending from the interior surface to the exterior surface;
- ii. a matrix material disposed on the exterior surface of the three-dimensional scaffold;
- iii. a culture medium disposed within the interior cavity and in fluidic contact with the interior surface;
- iv. a plurality of live plasma cells disposed on the matrix material;
- and
- v. a confluent monolayer of polarized mammary cells disposed on the plurality of plasma cells, wherein the mammary cells are selected from the group consisting of:
- live mammary epithelial cells, and live mammary myoepithelial cells, wherein the polarized mammary cells comprise an apical surface from which the milk product is secreted and a basal surface;
- said bioreactor comprising an apical compartment that is in fluidic contact with the apical surface of the live mammary cells, is substantially isolated from the interior cavity of the live cell construct, and is substantially free of cell culture medium;
- and
- b. isolating the milk product secreted into the apical compartment from the apical surface of the live mammary cells and the plasma cells.
8 more elements
A live cell construct, comprising:
- a. a three dimensional scaffold having an exterior surface, an interior surface defining an interior cavity/basal chamber, and a plurality of pores extending from the interior surface to the exterior surface;
- b. a matrix material disposed on the exterior surface of the three-dimensional scaffold;
- c. a plurality of live plasma cells disposed on the matrix material;
- d. an at least 70% confluent monolayer of live polarized mammary cells disposed on the plurality of live plasma cells and on the matrix material, wherein the live mammary cells are selected from the group consisting of:
- live mammary epithelial cells, and live mammary myoepithelial cells, and wherein the live polarized mammary cells comprise an apical surface from which the milk product is secreted and a basal surface;
- and
- e. a culture medium disposed within the interior cavity/basal chamber and in fluidic contact with the interior surface, wherein the culture medium is substantially isolated from the apical surface of the live mammary cells.
4 more elements
Dependencies are inferred from the claim text.