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US 11,199,540 B2Granted
Reversible surface functionalization
AssigneeIllumina Cambridge Limited
InventorsXavier von Hatten, Wayne N. George, Alexandre Richez, Anne-Cecile Dingwall, Andrew A. Brown
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Abstract
Some examples described herein relate to a substrate comprising a silane functionalized surface for reversibly immobilizing a biological molecule of interest, such as oligonucleotides, polynucleotides, or protein. Methods for immobilizing the biological molecule and the use in DNA sequencing and other diagnostic applications are also disclosed.
Claims12
A method for reversibly immobilizing a biological molecule to a surface of a substrate, comprising:
- contacting a plurality of host molecules with functionalized silane, wherein the functionalized silane comprises a plurality of hydrophobic guest moieties, the plurality of hydrophobic guest moieties comprise aryl, heteroaryl, heterocyclyl, cycloalkenyl, or cycloalkynyl, or optionally substituted variants thereof, and the functionalized silane is covalently attached to the surface of the substrate;
- and wherein the host molecules are optionally substituted cyclodextrin comprising a hydrophilic portion and a hydrophobic portion;
- wherein each host molecule comprises a biological molecule covalently bonded to the hydrophilic portion of the host molecule;
- and
- wherein the biological molecule is immobilized to the surface of the substrate through a host-guest reversible interaction between the hydrophobic guest moiety of the functionalized silane and the hydrophobic portion of the host molecule.
2 more elements
Dependencies are inferred from the claim text.