Biotransplant for treating gum recession and restoring gingival tissue volume

A method of treatment of a periodontal tissue, comprising:

• introducing a biotransplant into a damaged part of the gingiva using a surgery, wherein the biotransplant is obtained by a method comprising:
• introducing autologous fibroblasts isolated from an oral mucosa of a patient into a platelet-rich fibrin (PRF) membrane, thereby producing the biotransplant,
• wherein the autologous fibroblasts are suspended in a suitable medium at a concentration of from 5×106 to 20×106 cells/ml,
• wherein the introducing comprises linear retrograde needle injection of the suspended autologous fibroblasts into the PRF membrane,
• wherein the retrograde needle injection comprises inserting a needle into a thickness of the PRF membrane and making a first puncture and then a series of punctures linearly aligned or arrayed with the first puncture each spaced at a distance of 0.2 to 0.33 mm from a prior puncture, and
• wherein an amount of from 50 to 100 μL of a 1 ml suspension of the autologous fibroblasts is injected into each puncture until an entire amount of the 1 ml suspension has been injected into the punctures.

A method for treating a periodontal tissue comprising grafting a biotransplant over a periodontal tissue of a subject in need thereof, wherein the biotransplant is obtained by a method comprising:

• introducing autologous fibroblasts isolated from an oral mucosa of a patient into a platelet-rich fibrin (PRF) membrane, thereby producing the biotransplant,
• wherein the autologous fibroblasts are suspended in a suitable medium at a concentration of from 5×106 to 20×106 cells/ml,
• wherein the introducing comprises linear retrograde needle injection of the suspended autologous fibroblasts into the PRF membrane,
• wherein the retrograde needle injection comprises inserting a needle into a thickness of the PRF membrane and making a first puncture and then a series of punctures linearly aligned or arrayed with the first puncture each spaced at a distance of 0.2 to 0.33 mm from a prior puncture, and
• wherein an amount of from 50 to 100 μL of a 1 ml suspension of the autologous fibroblasts is injected into each puncture until an entire amount of the 1 ml suspension has been injected into the punctures.

A biotransplant obtained by a method comprising:

• introducing autologous fibroblasts isolated from an oral mucosa of a patient into a platelet-rich fibrin (PRF) membrane, thereby producing the biotransplant,
• wherein the autologous fibroblasts are suspended in a suitable medium at a concentration of from 5×106 to 20×106 cells/ml,
• wherein the introducing comprises linear retrograde needle injection of the suspended autologous fibroblasts into the PRF membrane,
• wherein the retrograde needle injection comprises inserting a needle into a thickness of the PRF membrane and making a first puncture and then a series of punctures linearly aligned or arrayed with the first puncture each spaced at a distance of 0.2 to 0.33 mm from a prior puncture, and
• wherein an amount of from 50 to 100 μL of a 1 ml suspension of the autologous fibroblasts is injected into each puncture until an entire amount of the 1 ml suspension has been injected into the punctures.