Recombinant yeast cell
AssigneeDsm IP Assets B.V.
InventorsIoannis Papapetridis, Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Paulus Petrus De Waal, Niels Lauret, Maarten Dirk Verhoeven, Jozef Petrus Johannes Schmitz
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Abstract
The present invention describes a recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39; Rubisco), and optionally one or more molecular chaperones for Rubisco, and one or more phosphoribulokinase (EC2.7.1.19; PRK), wherein the phosphoribulokinase is under control of a promoter (the “PRK promoter”) that enables higher expression under anaerobic conditions than under aerobic conditions.
Claims17
A recombinant yeast cell functionally expressing one or more heterologous nucleic acid sequences encoding for ribulose-1,5-phosphate carboxylase/oxygenase (EC4.1.1.39;
- Rubisco), and one or more phosphoribulokinase (EC2.7.1.19;
- PRK), wherein the phosphoribulokinase is under control of a promoter or a PRK promoter which has a PRK expression ratio anaerobic/aerobic of 2 or more,
- wherein the PRK promoter is the native promoter of a gene selected from the group consisting of:
- FET4 (FErrous Transport;
- YMR319C), ANB1 (ANaeroBically induced;
- YJR047C), YHK8 (YHR048W), DAN1 (Delayed ANaerobic;
- YJR150C), AAC3 (ADP/ATP Carrier;
- YBR085W), TIR2 (TIp1-Related;
- YOR010C), DIP5 (DIcarboxylic amino acid Permease;
- YPL265W), HEM13 (HEMe biosynthesis;
- YDR044W), YNR014W, YAR028W, FUN 57, COX5B (Cytochrome c OXidase;
- YIL111W), OYEZ (Old Yellow Enzyme;
- YHR179W), SUR2 (SUppressor of Rvs161 and rvs167 mutations;
- YDR297W), FRDS1 (Fumarate ReDuctase;
- YEL047C), PIS1 (Phosphatidyl Inositol Synthase;
- YPR113W), LAC1 (Longevity-Assurance gene Cognate (LAG1 Cognate);
- YKL008C), YGR035C, FRT2 (Functionally Related to TCP1;YAL028W), EUG1 (ER protein Unnecessary for Growth;
- YDR518W), HEM14 (HEMe biosynthesis;
- YER014W), ISU2 (IscU homolog;
- YOR226C), ERG26 (ERGosterol biosynthesis;
- YGL001C), MLO01 (Mitochondrially LOcalized protein;
- YMR252C), SML1 (Suppressor of Mec1 Lethality;
- YML058W) TIR4 (YOR009W), TIR3 (YIL011W), PAU7 (YAR020C), PAU5 (YFL020C), YLL064C, YGR294W, DAN3 (YBR301W;
- PAU24), YIL176C, YGL261C, YOL161C, PAU1 (YJL223C), PAU6 (YNR076W), DAN2 (YLR037C;
- PAU23), YDR542W, YIR041W, YKL224C, PAU3 (YCR104W), YLL025W, YOR394W, YHL046C, YMR325W, YAL068C, YPL282C, PAU2 (YEL049W), and PAU4 (YLR461W).
Dependencies are inferred from the claim text.