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US 11,185,838 B2Granted
Neutralization and containment of redox species produced by circumferential electrodes
AssigneeCustomArray, Inc.
InventorsKarl Maurer, John J Cooper, Jr., H Sho Fuji, Joseph Leonetti
Life of this patent
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Abstract
There is disclosed an electrode array architecture employing continuous and discontinuous circumferential electrodes. There is further disclosed a process for the neutralization of acid generated at anode(s) by base generated at cathode(s) circumferentially located to each other so as to confine a region of pH change. The cathodes can be displayed as concentric rings (continuous) or as counter electrodes in a cross pattern (discontinuous). In this way reagents, such as acid, generated in a center electrode are countered (neutralized) by reagents, such as base, generated at the corners or at the outer ring.
Claims20
An oligonucleotide bound to a semiconductor electrode array comprising:
- an oligouncleotide bound to a porous reaction layer adsorbed to and forming a layer on a top surface of a semiconductor electrode array, where the semiconductor electrode array comprises a plurality of continuous circumferential electrodes, where each of the plurality of continuous circumferential electrodes is separately addressable, where each electrode of the plurality of continuous circumferential electrodes comprises:
- (a) a top conducting surface region, where the top conducting surface region is defined by a top conducting surface which makes up a part of the top surface;
- (b) an insulating material in a gap region, where the gap region is defined by an inner margin and an outer margin, where the inner margin borders with the insulating material that surrounds an anode of a first continuous circumferential electrode of the plurality of continuous circumferential electrodes and the outer margin is surrounded by a cathode of the first continuous circumferential electrode, where the anode is adapted to produce an acid, and where the cathode is adapted to produce a base;
- and
- (c) the porous reaction layer formed from polysaccharide material and monosaccharide material that adsorbs to the top conducting surface and contains free hydroxyl groups or free amine groups or sulfhydryl groups (or combinations thereof) for covalently binding the oligonucleotide, wherein the porous reaction layer forms a layer over the plurality of continuous circumferential electrodes.
2 more elements
An oligonucleotide bound to a semiconductor electrode array comprising:
- an oligouncleotide bound to a porous reaction layer formed from polysaccharide material and monosaccharide material adsorbed to a semiconductor electrode array, where the semiconductor electrode array comprises a plurality of continuous circumferential electrodes, where each of the plurality of continuous circumferential electrodes is independently separately addressable, where the plurality of continuous circumferential electrodes comprise:
- (a) an anode;
- (b) a cathode;
- and
- (c) an insulating material in a gap region, where the gap region is defined by an inner margin and an outer margin, where the inner margin borders with the anode, where the outer margin is surrounded by the cathode.
2 more elements
An oligonucleotide bound to a semiconductor electrode array comprising:
- an oligouncleotide bound to a porous reaction layer adsorbed to and forming a layer on a top surface of a semiconductor electrode array, where the porous reaction layer comprises a monosaccharide and a polysaccharide, where the semiconductor electrode array comprises:
- (a) a plurality of continuous circumferential electrodes;
- and
- (b) an insulating material, where each of the plurality of continuous circumferential electrodes comprises:
- (i) a top conducting surface, where the top conducting surface makes up a part of the top surface;
- (ii) an anode and a cathode, where the anode and the cathode are independently separately addressable;
- (iii) an insulating material;
- (iv) an inner margin and an outer margin, where the inner margin borders with the insulating material that surrounds at least one continuous circumferential electrode and the outer margin is surrounded by the insulating material, where a voltage applied to the anode of one or more of the plurality of continuous circumferential electrodes generates an acid to initiate a synthesis, and where a voltage applied to the cathode of one or more of the plurality of continuous circumferential electrodes generates a base to contain the acid produces a base to neutralize and thereby contain the acid;
- and
- (v) the porous reaction layer formed from polysaccharide material and monosaccharide material that adsorbs to the top surface and contains free hydroxyl groups or free amine groups or sulfhydryl groups (or combinations thereof) for covalently binding the oligonucleotide, wherein the porous reaction layer forms a layer over the semiconductor electrode array.
7 more elements
Dependencies are inferred from the claim text.