Back to results
US 11,181,537 B2Granted
Mass spectrometric determination of eicosapentaenoic acid and docosahexaenoic acid
AssigneeQuest Diagnostics Investments Incoporated
InventorsScott M. Goldman, Julie A. Neidich
Life of this patent
Estimating the term…
Abstract
The invention relates to the detection of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA). In a particular aspect, the invention relates to methods for detecting DHA and EPA by mass spectrometry and kits for carrying out such methods.
Claims14
A method for determining an amount of docosahexaenoic acid (DHA) and/or eicosapentaenoic acid (EPA) in a sample by mass spectrometry, the method comprising:
- (i) hydrolyzing the sample containing DHA and/or EPA with an acid;
- (ii) extracting DHA and/or EPA from the sample by liquid-liquid extraction;
- (iii) ionizing DHA and/or EPA from the sample to generate one or more ions detectable by mass spectrometry;
- (iv) determining an amount of DHA and/or EPA ions by single mass spectrometry;
- and
- (v) relating the amount of DHA and/or EPA ions to the amount of DHA and/or EPA in the sample, wherein a limit of detection of the method for DHA is between about 1 μmol/L and 0.25 μmol/L, and a limit of detection of the method for EPA is between about 1 μmol/L and 0.67 μmol/L.
3 more elements
Dependencies are inferred from the claim text.