Sample preparation device and methods of use
AssigneeCubed Laboratories, LLC
InventorsLeslie T. Ivie, Christopher Chanelli, Shaunasee Kocen
Life of this patent
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Abstract
A device for isolating DNA from a sample containing cells, including a cartridge having an entrance port and an exit port, a membrane disposed between the entrance port and the exit port, and a plurality of channels between the membrane and the exit port. Additionally, systems and methods for isolating DNA from a sample containing cells and also systems and methods for amplifying and isolating single-stranded DNA from a sample containing DNA.
Claims17
A method of amplifying and isolating single-stranded DNA from a sample containing DNA, comprising
- providing an amplification container containing a magnetic substrate and a sample containing DNA;
- wherein the magnetic substrate has a coating comprising N-hydroxysuccinimide (NHS);
- amplifying the DNA in the amplification container via a polymerase chain reaction (PCR), wherein the PCR comprises asymmetric PCR, to produce a mixture of double-stranded DNA and single-stranded DNA;
- binding the magnetic substrate to the double-stranded DNA;
- wherein the magnetic substrate has an affinity for binding the double-stranded DNA;
- applying a magnetic field to the magnetic substrate, wherein the magnetic field attracts the magnetic substrate bound to the double-stranded DNA;
- and
- isolating the single-stranded DNA from the mixture.
A method of amplifying and isolating single-stranded DNA from a sample containing DNA, comprising
- providing an amplification container containing a magnetic substrate and a sample containing DNA;
- wherein the magnetic substrate has a coating comprising a carboxylic acid linker;
- amplifying the DNA in the amplification container via a polymerase chain reaction (PCR), wherein the PCR comprises asymmetric PCR, to produce a mixture of double-stranded DNA and single-stranded DNA;
- binding the magnetic substrate to the double-stranded DNA;
- wherein the magnetic substrate has an affinity for binding the double-stranded DNA;
- applying a magnetic field to the magnetic substrate, wherein the magnetic field attracts the magnetic substrate bound to the double-stranded DNA;
- and
- isolating the single-stranded DNA from the mixture.
A method of amplifying and isolating single-stranded DNA from a sample containing DNA, comprising
- providing an amplification container containing a plurality of silica-based magnetic beads and a sample containing DNA, wherein the magnetic beads have a coating comprising N-hydroxysuccinimide (NETS);
- amplifying the DNA in the amplification container via a polymerase chain reaction (PCR), wherein the PCR comprises asymmetric PCR, to produce a mixture of double-stranded DNA and single-stranded DNA;
- binding the magnetic beads to the double-stranded DNA during asymmetric PCR;
- applying a magnetic field to the magnetic beads, wherein the magnetic field attracts the magnetic beads bound to the double-stranded DNA;
- and
- isolating the single-stranded DNA from the mixture.
Dependencies are inferred from the claim text.