Single cell-derived organoids
AssigneeRutgers, The State University of New Jersey
InventorsHatem Sabaawy, Monica Bartucci
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Abstract
The present invention relates to organoids derived from a single cell, such as a prostate cancer cell, and methods and compositions relating to the production and use thereof, including cell culture medium for producing organoids and methods of personalized treatment for prostate cancer. The invention further provides a humanized mouse comprising a prostate organoid derived from a patient's prostate cell.
Claims22
A prostate organoid clonally derived in vitro from a single stem cell from primary prostate cancer tissue, wherein the organoid comprises epithelial cells and an outer layer of cells of a mesenchymal lineage surrounded by a basement membrane.
A prostate organoid clonally derived from a single stem cell made by the method of:
- isolating cells from a human prostate tissue to provide isolated cells;
- culturing the isolated cells for a time sufficient to enrich for stem cells;
- and amplifying a single stem cell by culturing in a three-dimensional culture comprising an extracellular matrix in an organoid medium for a time sufficient to produce at least one organoid, wherein the organoid medium comprises basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) and hydrocortisone,
- the at least one organoid clonally derived from the single stem cell and comprising epithelial cells and an outer layer of cells of a mesenchymal lineage surrounded by a basement membrane.
Dependencies are inferred from the claim text.