In-vitro full-skin model containing three-dimensional cell culture models of the sweat gland

A method for the production of an in-vitro full skin model, wherein the method comprises the following steps in the specified sequence:

• a) providing a suspension of low-solubility collagen,
• b) producing a supporting layer by freeze-drying the collagen suspension provided in step a),
• c) producing a dermal equivalent by applying primary fibroblasts to the supporting layer produced in step b) and culturing the fibroblasts over a time period of from about 7 to about 28 days,
• d) applying primary keratinocytes to the dermal equivalent produced in step c) and culturing the keratinocytes over a time period of from about 1 to about 10 days,
• e) culturing the model obtained after step d) at the air-medium boundary over a time period of from about 7 to about 42 days,
• wherein from about 1 to about 100 three-dimensional sweat gland equivalents with a cell count of respectively from about 500 to about 500000 sweat gland cells and a diameter of respectively from about 100 to about 6000 μm are introduced by adding the equivalents in step c) and/or step d);
• wherein the three-dimensional sweat gland equivalents are free from matrix compounds and supports;
• and
• wherein the three-dimensional sweat gland equivalents are three-dimensional sweat gland equivalents of the eccrine and/or apocrine human sweat gland.