A cyclic chromatographic purification method for the isolation of a product from a feed mixture comprising the product and at least one further component representing impurities, which impurities bind stronger to a chromatographic stationary phase than the product,
- the method using at least two chromatographic non-affinity adsorbers as a chromatographic stationary phase, said at least two chromatographic non-affinity adsorbers being grouped into only one first adsorber section and one second adsorber section, there being no adsorber sections used other than said first and second adsorber sections,
- wherein if either of said only two adsorber sections comprises more than one chromatographic adsorber, said more than one chromatographic adsorber in an adsorber section are permanently connected in series,
- wherein the first adsorber section has a first adsorber section inlet and a first adsorber section outlet, and the second adsorber section has a second adsorber section inlet and a second adsorber section outlet,
- the method comprising the following steps in order:
- a. a first interconnected step,
- wherein the first adsorber section outlet is connected to the second adsorber section inlet during a first interconnected timespan,
- wherein the first adsorber section is loaded via the first adsorber section inlet with the feed mixture, and
- wherein the product is collected from the second adsorber section outlet;
- b. a second interconnected step conducted directly after said first interconnected step without disconnection,
- wherein the first adsorber section outlet is connected to the second adsorber section inlet during a second interconnected timespan,
- wherein the first adsorber section is loaded via the first adsorber section inlet with a first washing buffer to transfer the product unbound in the first absorber section into the second adsorber section,
- wherein a stream exiting the first adsorber section outlet is either:
- diluted inline before entering the second adsorber section inlet, or
- supplemented with the feed mixture, and
- wherein the product is collected from the second adsorber section outlet;
- c. a first batch step,
- wherein during a batch timespan said first and second adsorber sections are disconnected,
- wherein the first adsorber section is cleaned and regenerated to remove the impurities and the first adsorber section outlet is directed to waste,
- wherein the second adsorber section inlet is either:
- loaded with a second washing buffer, or
- loaded with the feed mixture, and
- wherein the product is collected from the second adsorber section outlet;
- d. a third interconnected step,
- wherein the first adsorber section performs tasks of the second adsorber section in the first interconnected step, and the second adsorber section performs tasks of the first adsorber section in the first interconnected step;
- e. a fourth interconnected step conducted directly after said third interconnected step without disconnection,
- wherein the first adsorber section performs tasks of the second adsorber section in the second interconnected step, and the second adsorber section performs tasks of the first adsorber section in the second interconnected step;
- and
- f. a second batch step,
- wherein the first adsorber section performs tasks of the second adsorber section in the first batch step, and the second adsorber section performs tasks of the first adsorber section in the first batch step.