US 11,179,723 B2 · System, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells

Abstract

A cell processing system, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells are disclosed. Particularly, the cell processing system includes a fluidics cartridge and a control instrument. The fluidics cartridge includes a cell processing chamber that has a micropost array therein, a sample reservoir and a wash reservoir that supply the cell processing chamber, and a waste reservoir and an eluent reservoir at the output of the cell processing chamber. A micropost actuation mechanism and a cell counting mechanism are provided in close proximity to the cell processing chamber. A method is provided of using the cell processing system to collect, wash, and recover cells. Another method is provided of using the cell processing system to collect, wash, count, and recover cells at a predetermined cell density.

Claims25

Dependent claims

A cell processing system comprising:

a fluidics cartridge comprising:
a cell processing chamber comprising a bottom substrate and a top substrate separated by the gap, wherein the cell processing chamber further comprises a micropost array, wherein the micropost array comprises a plurality of surface-attached microposts arranged on a micropost substrate, and wherein the micropost substrate is positioned atop the bottom substrate;
and
a control instrument;
wherein the surface-attached posts are configured for actuation in the presence of an actuation force, wherein no binding agents are disposed on or integrated with the surface-attached posts, the bottom substrate, the top substrate, or the micropost substrate, and wherein the bottom substrate and the top substrate are arranged atop a registration feature configured for mounting on the control instrument.

2 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/499,139Filed 23 Mar 2018 · us provisional application

Priority

28 Mar 2017

Published

US 2020/0368747 A126 Nov 2020

Granted

23 Nov 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification24 codes

B01L 3/502761
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
B01F 33/3038
Micromixers: using ciliary stirrers to move or stir the fluids
B01L 3/502746
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
C12M 23/16
Form or structure of the vessel: microfluidic devices; Capillary tubes

System, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells

Life of this patent

System, fluidics cartridge, and methods for using actuated surface-attached posts for processing cells

Life of this patent

Abstract

Claims25