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AssigneeEiken Kagaku Kabushiki Kaisha
InventorsRyota Yasui, Megumi Yui, Mitsuru Makinodan, Yasuhiro Seki, Chisa Sakanishi
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The present invention provides an anti-human hemoglobin monoclonal antibody or an antibody kit for specifically detecting and measuring a hemoglobin-haptoglobin complex in a sample with ease, and an insoluble carrier particle to which the monoclonal antibody is immobilized, and a measurement reagent and a measurement method for specifically detecting and measuring a hemoglobin-haptoglobin complex in a sample using the same. The anti-human hemoglobin monoclonal antibody of the present invention does not react to free hemoglobin or free haptoglobin which is not formed in a complex, but specifically reacts to a hemoglobin-haptoglobin complex when the antibody is immobilized to an insoluble carrier particle and used.
An antibody kit comprising monoclonal antibody-immobilized insoluble carrier particles, wherein one or more antibodies selected from the group consisting of 5C-2A deposited under NPMD accession number NITE BP-02268, 7C-7B-8E deposited under NPMD accession number NITE BP-02270, 1-5G-3C deposited under NPMD accession number NITE BP-02271, 12-9G-C deposited under NPMD accession number NITE BP-02269 and 79-8G-3F deposited under NPDM accession number NITE BP-02272, are immobilized on the insoluble carrier particles.
An anti-human hemoglobin monoclonal antibody, wherein the antibody is selected from the group consisting of 5C-2A deposited under NPMD accession number BP-02268, 7C-7B-8E deposited under NPMD accession number NITE-02270, 1-5G-3C deposited under NPMD accession number NITE BP-02271, 12-9G-C deposited under NPMD accession number NITE BP-02269, or 79-8G-3F deposited under NPMD accession number NITE BP-02272.
An immunological agglutination measurement method for specifically detecting a hemoglobin-haptoglobin complex, the method comprising:
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