Method for detecting indicators for determining diseases

A method for the qualitative and/or quantitative determination of disease indicators in which a sample is tested for at least two aggregate types of endogenous misfolded proteins, wherein the sample is tested for at least two different aggregate types on the same substrate without further processing and/or treatment and the method comprises:

• (a) application of the sample to be tested to a substrate,
• (b) addition of probes labeled for detection with fluorescent dyes, which probes label a respective aggregate by specifically binding to it, and
• (c) detection of labeled aggregates by surface-based Fluorescence Intensity Distribution Analysis (sFIDA), carried out by a method with a temporally and spatially resolved signal and with high spatial resolution wherein a pixel is determined against a respective background, and wherein
• (b) can be carried out before (a);
• the disease is selected from tauopathies, AA (amyloid A) amyloidosis, AL (amyloid light chain) amyloidosis, AApoAI (amyloid apolipoprotein AI) amyloidosis, (amyloid apolipoprotein AII) AApoAII amyloidosis, ATTR (amyloid transthyretin) amyloidosis, schizophrenia and other DISC1 (disrupted in schizophrenia 1) opathies, amyotrophic lateral sclerosis, frontotemporal lobar degeneration and other FUS (fused in sarcoma) proteinopathies, diabetes mellitus type 2, Parkinson's disease and other synucleinopathies, chronic traumatic encephalopathy and other TDP-43 (transactive response DNA binding protein 43 kDa) proteinopathies, Huntington's disease, familial visceral amyloidosis and/or Alzheimer's dementia;
• and
• the aggregate type of endogenous misfolded proteins is selected from tau aggregates, serum amyloid A protein aggregates, IgG (immunoglobulin G) light chain aggregates, AapoAI aggregates, AapoAll aggregates, ATTR aggregates, DISC1 aggregates, FUS aggregates, IAPP (islet amyloid polypeptide) aggregates, SOD1 (superoxide dismutase 1) aggregates, α-synuclein aggregates, TDP-43 aggregates, huntingtin aggregates, lysozyme aggregates, Aβ aggregates, and mixed aggregates.