Electrochemical assay device and related methods
AssigneeAgaMatrix, Inc.
InventorIan Harding
Life of this patent
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Abstract
An electrochemical test device is provided having a base layer with a first electrode thereon and a top layer with a second electrode thereon. The two electrodes are separated by a spacer layer having an opening therein, such that a sample-receiving space is defined with one electrode on the top surface, the other electrodes on the bottom surface and side walls formed from edges of the opening in the spacer. Reagents for performing the electrochemical reaction are deposited on one of the electrodes and on the side walls of the sample-receiving space.
Claims21
An electrochemical test device comprising
- a first substrate having a first electrode disposed thereon, a second substrate having a second electrode disposed thereon, and a spacer disposed between the first and second substrates and having an opening therein, whereby a sample-receiving space is defined that has a first surface having the first electrode disposed thereon, a second surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer;
- and
- a reagent comprising a redox active material which is oxidized at the first electrode and reduced at the second electrode when the device is used,
- wherein the reagent is disposed in a dried layer, covering as a coating at least a portion of the first or second electrode and at least a portion of the side walls, said layer extending as a coating contiguously from the covered electrode and along the side walls.
A method of making an electrochemical test device comprising the steps of:
- (a) forming a first substrate having a first electrode disposed thereon;
- (b) forming a spacer layer on the first substrate, said spacer layer having an opening formed therein through which the first electrode is exposed and side walls within the opening;
- (c) introducing a liquid reagent comprising a redox active material into the opening in the spacer layer,
- (d) drying the liquid reagent to form a dried reagent, wherein the liquid reagent is introduced to the opening in such a manner that upon drying a dried reagent layer is formed contiguously covering at least a portion of the first electrode and at least a portion of the side walls;
- and
- (e) placing a second substrate having a second electrode disposed thereon on the spacer layer aligned to have the second electrode facing the first electrode, thereby forming a sample-receiving space that has a first surface having the first electrode disposed thereon, a second surface, opposite the first surface, having the second electrode disposed thereon, and side walls formed from edges of the opening in the spacer.
Dependencies are inferred from the claim text.