US 11,174,499 B2Granted

Enzyme compositions and uses thereof

AssigneeNovozymes A/S

InventorsJeffrey Shasky, Debbie Yaver, Donna Moyer

Life of this patent

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Abstract

The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.

Claims18

Dependent claims

A recombinant fungal host cell, comprising polynucleotides encoding an enzyme composition comprising:

a cellobiohydrolase I, a cellobiohydrolase II, a beta-glucosidase variant comprising one or more substitutions selected from the group consisting of F100D, S283G, N456E, and F512Y of SEQ ID NO:
6 or the mature polypeptide thereof, an AA9 polypeptide having cellulolytic enhancing activity, a GH10 xylanase, and a beta-xylosidase;
wherein the cellobiohydrolase I is selected from the group consisting of:
(i) a cellobiohydrolase I comprising or consisting of the mature polypeptide of SEQ ID NO:
2;
(ii) a cellobiohydrolase I comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
2;
(iii) a cellobiohydrolase I encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
1;
and (iv) a cellobiohydrolase I encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
1;
wherein the cellobiohydrolase II is selected from the group consisting of:
(i) a cellobiohydrolase II comprising or consisting of the mature polypeptide of SEQ ID NO:
4;
(ii) a cellobiohydrolase II comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
4;
(iii) a cellobiohydrolase II encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
3;
and (iv) a cellobiohydrolase II encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
3;
wherein the xylanase is selected from the group consisting of:
(i) a xylanase comprising or consisting of the mature polypeptide of SEQ ID NO:
10 or the mature polypeptide of SEQ ID NO:
12;
(ii) a xylanase comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
10 or the mature polypeptide of SEQ ID NO:
12;
(iii) a xylanase encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
9 or the mature polypeptide coding sequence of SEQ ID NO:
11;
and (iv) a xylanase encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
9 or the mature polypeptide coding sequence of SEQ ID NO:
11;
and
wherein the beta-xylosidase is selected from the group consisting of:
(i) a beta-xylosidase comprising or consisting of the mature polypeptide of SEQ ID NO:
14;
(ii) a beta-xylosidase comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
14;
(iii) a beta-xylosidase encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
13;
and (iv) a beta-xylosidase encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
13.

40 more elements

Dependencies are inferred from the claim text.

Facts

Application: 16/748,446Filed 21 Jan 2020 · division
Priority: 6 Jun 2014
Published: US 2020/0151397 A114 May 2020
Granted: 16 Nov 2021
Term ends: Not estimatedThe source records don’t support an estimate.

Classification24 codes

C12P 19/14
Preparation of compounds containing saccharide radicals: produced by the action of a carbohydrase , e.g. by alpha-amylase , e.g. by cellulase,…
C12N 9/0004
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: oxidoreductases
C12N 9/0065
Oxidoreductases: acting on hydrogen peroxide as acceptor
C12N 9/24
Hydrolases: acting on glycosyl compounds

Family

Application62/009,018Us provisional application · filed 6 Jun 2014
Application62/093,230Us provisional application · filed 17 Dec 2014
PCT/US2015/034179Parent of this division · filed 4 Jun 2015
Application16/386,660Parent of this division · filed 17 Apr 2019
US 2020/0151397 A1Pre-grant publication · published 14 May 2020
Application15/316,477Parent of this division
US 10,273,515 B2Parent of this division
US 11,334,720 B2Related patent
US 2017/0204441 A1Published application
US 2020/0334331 A1Published application
US 11,174,499 B2This patent

From the US filing history. Foreign family members aren’t listed.

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Abstract

The present invention relates to enzyme compositions and processes of producing and using the compositions for the saccharification of lignocellulosic material.

A recombinant fungal host cell, comprising polynucleotides encoding an enzyme composition comprising:

a cellobiohydrolase I, a cellobiohydrolase II, a beta-glucosidase variant comprising one or more substitutions selected from the group consisting of F100D, S283G, N456E, and F512Y of SEQ ID NO:
6 or the mature polypeptide thereof, an AA9 polypeptide having cellulolytic enhancing activity, a GH10 xylanase, and a beta-xylosidase;
wherein the cellobiohydrolase I is selected from the group consisting of:
(i) a cellobiohydrolase I comprising or consisting of the mature polypeptide of SEQ ID NO:
2;
(ii) a cellobiohydrolase I comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
2;
(iii) a cellobiohydrolase I encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
1;
and (iv) a cellobiohydrolase I encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
1;
wherein the cellobiohydrolase II is selected from the group consisting of:
(i) a cellobiohydrolase II comprising or consisting of the mature polypeptide of SEQ ID NO:
4;
(ii) a cellobiohydrolase II comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
4;
(iii) a cellobiohydrolase II encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
3;
and (iv) a cellobiohydrolase II encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
3;
wherein the xylanase is selected from the group consisting of:
(i) a xylanase comprising or consisting of the mature polypeptide of SEQ ID NO:
10 or the mature polypeptide of SEQ ID NO:
12;
(ii) a xylanase comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
10 or the mature polypeptide of SEQ ID NO:
12;
(iii) a xylanase encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
9 or the mature polypeptide coding sequence of SEQ ID NO:
11;
and (iv) a xylanase encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
9 or the mature polypeptide coding sequence of SEQ ID NO:
11;
and
wherein the beta-xylosidase is selected from the group consisting of:
(i) a beta-xylosidase comprising or consisting of the mature polypeptide of SEQ ID NO:
14;
(ii) a beta-xylosidase comprising or consisting of an amino acid sequence having at least 95% sequence identity to the mature polypeptide of SEQ ID NO:
14;
(iii) a beta-xylosidase encoded by a polynucleotide comprising or consisting of the mature polypeptide coding sequence of SEQ ID NO:
13;
and (iv) a beta-xylosidase encoded by a polynucleotide comprising or consisting of a nucleotide sequence having at least 95% sequence identity to the mature polypeptide coding sequence of SEQ ID NO:
13.

40 more elements

Facts

Application

16/748,446Filed 21 Jan 2020 · division

Priority

6 Jun 2014

Published

US 2020/0151397 A114 May 2020

Granted

16 Nov 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification24 codes

C12P 19/14
Preparation of compounds containing saccharide radicals: produced by the action of a carbohydrase , e.g. by alpha-amylase , e.g. by cellulase,…
C12N 9/0004
Enzymes; Proenzymes; Compositions thereof ; Processes for preparing, activating, inhibiting, separating or purifying enzymes: oxidoreductases
C12N 9/0065
Oxidoreductases: acting on hydrogen peroxide as acceptor
C12N 9/24
Hydrolases: acting on glycosyl compounds

Family

Application62/009,018Us provisional application · filed 6 Jun 2014

Application62/093,230Us provisional application · filed 17 Dec 2014

PCT/US2015/034179Parent of this division · filed 4 Jun 2015

Application16/386,660Parent of this division · filed 17 Apr 2019

US 2020/0151397 A1Pre-grant publication · published 14 May 2020

Application15/316,477Parent of this division

US 10,273,515 B2Parent of this division

US 11,334,720 B2Related patent

US 2017/0204441 A1Published application

US 2020/0334331 A1Published application

US 11,174,499 B2This patent

From the US filing history. Foreign family members aren’t listed.