Genetically modified acetogenic cell
AssigneeEvonik Operations GmbH
InventorsThomas Haas, Thomas Bülter, Anja Hecker
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Abstract
There is provided an acetogenic microbial cell which is capable of producing at least one higher alcohol from a carbon source, wherein the acetogenic microbial cell is genetically modified to comprise an increased expression relative to its wild type cell of at least one enzyme, E8, a butyryl-CoA:acetate CoA transferase (cat3). There is also provided a method and use of the cell to produce higher alcohols.
Claims16
An acetogenic microbial cell which is capable of producing at least one higher alcohol from a carbon source, wherein the acetogenic microbial cell is genetically modified to comprise at least one enzyme, E8, a butyryl-CoA:
- acetate CoA transferase (cat3), wherein said E8 comprises an amino acid sequence having at least 100% sequence identity with the amino acid sequence of SEQ ID NO:1 of an enzyme obtained from Clostridium kluyveri, and wherein the higher alcohol comprises the structure of formula I below and has 4 to 10 carbon atoms:
- wherein the acetogenic microbial cell is Clostridium ljungdahlii or Clostridium autothenogenum.
An acetogenic microbial cell which is capable of producing at least one higher alcohol from a carbon source, wherein:
- the acetogenic microbial cell is genetically modified to comprise increased butyryl-CoA:
- acetate CoA transferase (cat3 activity) relative to the acetogenic microbial cell before being genetically modified, said increased activity being at least partially due to the enzyme E8, wherein said E8 comprises an amino acid sequence having at least 100% sequence identity with the amino acid sequence of SEQ ID NO:
- 1 of an enzyme of obtained from Clostridium kluyveri;
- the higher alcohol comprises the structure of formula I below and has 4 to 10 carbon atoms:
- wherein R=H or CH3, and n=1-6;
- and wherein the acetogenic microbial cell is Clostridium ljungdahlii or Clostridium autothenogenum.
Dependencies are inferred from the claim text.