Protein muNS that can form inclusions in the endoplasmic reticulum, methods for the use thereof and uses of same

A product selected from the group consisting of:

• (a) a polynucleotide encoding a polypeptide comprising:
• (i) a first component comprising a secretory pathway signal peptide, and
• (ii) a second component comprising the minimum region of an Orthoreovirus muNS protein set forth in the amino acid sequence of SEQ ID NO:
• 11 or SEQ ID NO:
• 12 or a functionally equivalent variant thereof having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
• 11 or SEQ ID NO:
• 12, wherein said second component has the capacity to form inclusions in the endoplasmic reticulum when expressed in a cell, and wherein said second component lacks N-glycosylation consensus sequences,
• wherein said first component is located in the N-terminal position with respect to said second component;
• (b) a polypeptide encoded by the polynucleotide of (a);
• (c) a polynucleotide encoding a fusion protein comprising:
• (i) a first component comprising a secretory pathway signal peptide,
• (ii) a second component comprising a polypeptide of interest, and
• (iii) a third component comprising the minimum region of an Ortheovirus muNS protein set forth in the amino acid sequence of SEQ ID NO:
• 21-24 or a functionally equivalent variant thereof having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:
• 21-24, wherein said third component has the capacity to incorporate itself into inclusions formed in the endoplasmic reticulum by a protein comprising the minimum region of an Orthoreovirus muNS having the capacity to form inclusions in the endoplasmic reticulum when expressed in a cell, and wherein said third component lacks N-glycosylation consensus sequences,
• wherein said first component is located in the N-terminal position with respect to said second and third components;
• and
• (d) a fusion protein encoded by the polynucleotide of (c).