Galactose utilization
AssigneeWilliam Marsh Rice University
InventorsKa-Yiu San, Ping Liu, Sha Li
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Abstract
The present disclosure describes a genetically engineered bacteria that relieves the catabolite repression problem exerted by the Spot 42 small regulatory RNA by adding a galactokinase that does not contain the Spot 42 binding region. As such, galK and galM and the like can be expressed allow better galactose utilization.
Claims18
A recombinant bacteria, said bacteria being from a gammaproteobacterial species having a Spot 42 (spf) responsive galactokinase (GalK) when said species is wild type at GalK, and said bacteria comprising:
- a) a knockout mutation of an endogenous spot 42 responsive GalK;
- and
- b) expressing an open reading frame (ORF) from a GalK lacking a Spot 42 binding region (GalKSpot42-) under the control of a promotor that is not native to the GalKSpot42-;
- wherein said recombinant bacteria is able to avoid catabolite repression and grow on mixed sugars comprising galactose and glucose.
A recombinant bacteria, said bacteria being a gammaproteobacterial species having a Spot 42 (spf) responsive GalK when said species is wild type at GalK, and said recombinant bacteria comprising:
- a) a knockout mutation of an endogenous spot 42 responsive GalK;
- and
- b) expressing a GalKSpot42- ORF from Bifidobacterium under a promoter that is not native to Bifidobacterium;
- wherein said bacteria is able to avoid catabolite repression and grow on mixed sugars comprising galactose and glucose.
Dependencies are inferred from the claim text.