Immune modulation
AssigneeSementis Limited
InventorPaul Michael Howley
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Abstract
The present invention relates to a poxvirus vector comprising a nucleic acid sequence encoding a fusion protein comprising: (i) a peanut allergen selected from list consisting of at least two peanut allergens from ara h 1, ara h 2, ara h 3, ara h 4, ara h 5, ara h 6. ara h 7, ara h 8, ara h 9, ara h I O and ara h I I or a derivative or part thereof having at least 70% sequence identity thereto, and (ii) a profeasome degradation tag to enhance intracellular degradation of the fusion protein. Methods of desensitizing or inducing tolerance to a peanut allergen and/or suppressing an allergic response to a peanut allergen are also disclosed.
Claims15
A vaccinia virus vector comprising a nucleic acid sequence encoding a fusion protein, comprising:
- (i) a proteasome degradation tag to enhance intracellular degradation of the fusion protein;
- and
- (ii) peanut allergens:
- ara h 1 comprising the sequence of amino acids set forth as SEQ ID NO:4,
- ara h 2 comprising the sequence of amino acids set forth as SEQ ID NO:6,
- ara h 3 comprising the sequence of amino acids set forth as SEQ ID NO:8, and
- ara h 6 comprising the sequence of amino acids set forth as SEQ ID NO:10,
- wherein the fusion protein, when expressed from the vector and degraded, results in T-cell epitope-containing peptides that can complex with MHC class I proteins for presentation to T lymphocytes to produce a biased TH1 immune response to the T-cell epitope containing peptides.
A poxvirus vector, comprising a nucleic acid sequence encoding a fusion protein that comprises the amino acid sequence set forth in SEQ ID NO:12.
A vaccinia virus vector, comprising a nucleic acid sequence encoding a fusion protein comprising:
- (i) a proteasome degradation tag to enhance intracellular degradation of the fusion protein;
- and
- (ii) only the following four peanut allergens:
- ara h 1 comprising the sequence of amino acids set forth as SEQ ID NO:4, ara h 2 comprising the sequence of amino acids set forth as SEQ ID NO:6, ara h 3 comprising the sequence of amino acids set forth as SEQ ID NO:8, and ara h 6 comprising the sequence of amino acids set forth as SEQ ID NO:10,
- wherein the fusion protein, when expressed from the vector and degraded, results in T-cell epitope-containing peptides that can complex with MHC class I proteins for presentation to T lymphocytes to produce a biased TH1 immune response to the T-cell epitope containing peptides.
Dependencies are inferred from the claim text.