Methods of detection and treatment for cardiovascular disease and foot wounds
AssigneeWashington University
InventorsMohamed Zayed, Clay Semenkovich
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Abstract
Among the various aspects of the present disclosure is the provision of a method of detection, treatment, and monitoring of cardiovascular disease or a foot wound by detection of a novel biomarker, Fatty Acid Synthase (FAS). Briefly, therefore, the present disclosure is directed to methods that allow for improved, noninvasive, and reliable diagnosis of these conditions, particularly in subjects suffering from Type 2 Diabetes (T2D).
Claims23
A method of detecting circulating Fatty Acid Synthase (FAS) enzymatic activity in a subject who has or is suspected of having a cardiovascular-related disease, disorder, or condition, comprising:
- providing a serum or plasma sample from the subject;
- and
- detecting a level of circulating FAS enzymatic activity using a nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) depletion assay in the serum or plasma sample,
- wherein the serum or plasma is in an amount sufficient for the circulating FAS enzymatic activity level to be detected in a NADPH depletion assay.
A method of diagnosing a cardiovascular-related disease, disorder, or condition comprising:
- providing a serum or plasma sample from a subject suspected of having a cardiovascular-related disease, disorder, or condition;
- detecting a circulating FAS enzymatic activity level using a NADPH depletion assay in the serum or plasma sample;
- and
- comparing the circulating FAS enzymatic activity level of the subject and a control sample;
- wherein,
- the subject is diagnosed with a cardiovascular-related disease, disorder, or condition if the circulating FAS enzymatic activity level in the subject is elevated compared to the circulating FAS enzymatic activity level in a control sample;
- and
- the serum or plasma is in an amount sufficient for the circulating FAS enzymatic activity level to be detected in a NADPH depletion assay.
Dependencies are inferred from the claim text.