Cationic oil-in-water emulsions
AssigneeGlaxosmithkline Biologicals SA
InventorsLuis Brito, Michelle Chan, Andrew Geall, Derek O'Hagan
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Abstract
This invention generally relates to cationic oil-in-water emulsions that contain high concentrations of cationic lipids and have a defined oil:lipid ratio. The cationic lipid can interact with the negatively charged molecule thereby anchoring the molecule to the emulsion particles. The cationic emulsions described herein are useful for delivering negatively charged molecules, such as nucleic acid molecules to cells, and for formulating nucleic acid-based vaccines.
Claims20
A method for preparing a composition comprising an RNA molecule complexed with a particle of a cationic oil-in-water emulsion, comprising:
- (i) providing an oil-in-water emulsion comprising particles that are dispersed in an aqueous continuous phase, wherein the average diameter of said particles is from about 80 nm to about 150 nm;
- wherein the average diameter does not change by more than 10% when the emulsion is stored at 4° C. for one month;
- wherein the emulsion comprises an oil and a cationic lipid, and wherein;
- a. the ratio of oil:lipid (mole:mole) in the oil-in-water emulsion is at least about 8:1 (mole:mole),
- b. the concentration of cationic lipid in said composition is at least about 1.25 mM, and
- c. the cationic lipid is not DC-Cholesterol;
- (ii) providing an aqueous solution comprising the RNA molecule;
- and
- (iii) combining the oil-in-water emulsion of (i) and the aqueous solution of (ii), thereby preparing the composition.
Dependencies are inferred from the claim text.