Compositions and methods
AssigneeDiagnostig Ltd
InventorsMark Stephen Baird, Christopher David Gwenin, Juma'a Raheem Najeem Al-Dulayymi, Mohsin Omar Mohammed
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Abstract
A method of determining whether an individual is infected with a mycobacterial disease, the method comprising: (a) providing a system which comprises an antigen; (b) contacting the system with a sample obtained from the individual; and (c) detecting the presence or absence of binding of a biomarker in the sample with the antigen; wherein the antigen is an arabinose ester of a mycolic acid or an analogue thereof.
Claims8
A method of determining whether an individual is infected with a mycobacterial disease, the method comprising:
- (a) providing a system which comprises a synthetic antigen which is an arabinose ester of a mycolic acid of formula (III):
- (M)x-(S)y-(M′)z (III)
- wherein x is from 1 to 6, y is from 1 to 12, z is from 0 to 10, each M and each M′ is independently a mycolic acid moiety including a β-hydroxy acid moiety and each S is a monosaccharide unit, provided that at least one S is an arabinose unit;
- (b) contacting the system with a sample obtained from the individual;
- and
- (c) detecting the presence or absence of binding of a biomarker in the sample with the synthetic antigen, wherein the biomarker is an antibody indicative of infection with or exposure to mycobacteria and wherein step (c) involves contacting the system with a composition comprising a secondary antibody configured to interact with the biomarker, wherein either:
- (i) the secondary antibody is carried by colloidal gold particles:
- or
- (ii) the secondary antibody is bound to an enzyme, wherein the method further comprises adding a composition comprising a colorimetric substrate to the system and wherein the colorimetric substrate undergoes a color change on reaction with the enzyme, and
- wherein step (c) includes detecting the presence of binding of the biomarker in the sample with the synthetic antigen when the secondary antibody interacts with the biomarker.
A kit for determining the presence or absence of a biomarker in a sample, the kit comprising:
- a system which comprises a synthetic antigen which is an arabinose ester of a mycolic-acid of formula (III):
- (M)x-(S)y-(M′)z (III)
- wherein x is from 1 to 6, y is from 1 to 12, z is from 0 to 10, each M and each M′ is independently a mycolic acid moiety including a β-hydroxy acid moiety and each S is a monosaccharide unit, provided that at least one S is an arabinose unit;
- and
- a composition comprising a secondary antibody configured to interact with the biomarker,
- wherein the biomarker is an antibody indicative of infection with or exposure to mycobacteria.
A composition comprising at least 90 wt % of a single compound selected from compounds having the formula A, B, C, D, E, F, G, H, or I:
Dependencies are inferred from the claim text.