Virus causing respiratory tract illness in susceptible mammals
AssigneeErasmus University Medical Center Rotterdam
InventorsJan Cornelius De Jong, Ronaldus Adrianus Maria Fouchier, Bernadetta Gerarda Van Den Hoogen, Albertus D. M. E. Osterhaus, Jan Groen
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Abstract
The invention relates to the field of virology. The invention provides an isolated essentially mammalian negative-sense single-stranded RNA virus (MPV) within the subfamily Pneumovirinae of the family Paramyxoviridae and identifiable as phylogenetically corresponding to the genus Metapneumovirus and components thereof.
Claims16
A kit for determining the presence of metapneumovirus (MPV) in a mammalian subject, the kit comprising:
- a DNA probe of at least 10 nucleotides that hybridizes to a target polynucleotide under stringent conditions,
- wherein the target polynucleotide comprises a sequence encoding a polypeptide that is at least 90% identical to SEQ ID NO:28, SEQ ID NO:32, or SEQ ID NO:39, or the complement of the sequence of the sequence encoding the polypeptide, and
- wherein the DNA probe does not y hybridize to a polynucleotide from avian pneumovirus (APV) under stringent conditions.
A method for detecting a human metapneumovirus infection in a mammal, the method comprising:
- detecting, in a sample from the mammal, the presence of a component of human metapneumovirus by reacting the sample with a DNA probe of at least 10 nucleotides in length that hybridizes to a target polynucleotide under stringent conditions,
- wherein the target polynucleotide comprises a sequence encoding a polypeptide that is at least 90% identical to SEQ ID NO:28, SEQ ID NO:32, or SEQ ID NO:39 or the complement of the sequence encoding the polypeptide, and
- wherein the DNA probe does not hybridize to a polynucleotide from avian pneumovirus (APV) under stringent conditions.
A method for classifying a virus as a human metapneumovirus, the method comprising:
- detecting, in a sample comprising the virus, the presence of a component of human metapneumovirus by reacting the sample with a DNA probe of at least 10 nucleotides in length that hybridizes to a target polynucleotide under stringent conditions, and
- classifying the sample as containing human metapneumovirus;
- wherein the target polynucleotide comprises a sequence encoding a polypeptide that is at least 77% identical to a sequence selected from the group consisting of SEQ ID NO:28, SEQ ID NO:32, or SEQ ID NO:39 or the complement of the sequence encoding the polypeptide.
Dependencies are inferred from the claim text.