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US 11,162,133 B2Granted
Detection of an amplification product using pH-sensitive dye
AssigneeNew England Biolabs, Inc.
InventorsYinhua Zhang, Nathan Tanner, Thomas C. Evans, Jr.
Life of this patent
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Abstract
Methods are provided for a rapid, low cost approach to monitoring an amplification reaction. This includes monitoring the progress of isothermal or PCR amplification reactions to completion using pH-sensitive dyes that are either colored or fluorescent. Compositions are described that include a mixture of a DNA polymerase, deoxyribonucleotide triphosphate and Tris buffer in the range of 1.5 mM Tris to 5 mM Tris or equivalent.
Claims7
A method for identifying a target nucleic acid in a sample comprising:
- (a) setting up a isothermal amplification reaction that comprises the sample, phenol red and 1.5 mM to 5 mM Tris or an equivalent thereof;
- (b) incubating the isothermal amplification reaction under isothermal amplification conditions;
- and, (c) after or during the incubation of (b), detecting by eye a change in color from red to yellow of the amplification reaction, wherein a color change of red to yellow indicates that the reaction comprises an amplification product.
Dependencies are inferred from the claim text.