Inducible expression of genes in algae
AssigneeSynthetic Genomics, Inc.
InventorsJohn Verruto, Jessica Weir
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Abstract
The present application provides novel algal regulatory elements including inducible nitrate/nitrite promoter sequences and terminator sequences. The application further discloses DNA constructs comprising these novel regulatory elements, and recombinant microorganisms comprising these regulatory elements. Methods of modifying, producing, and using the regulatory elements are also disclosed. Methods disclosed in the present application are suited for inducible expressions of genes, such as a transgene or a native gene in algal species.
Claims24
A genetically engineered alga comprising a DNA sequence comprising an algal nitrite reductase inducible promoter having at least 90% sequence identity to SEQ ID NO:
- 1 operably linked to a DNA of interest encoding a polypeptide or a functional RNA, wherein the DNA of interest encoding a polypeptide or a functional RNA is not regulated by the promoter in nature, and wherein the DNA molecule is integrated into the algal genome.
An expression cassette comprising DNA molecule comprising an algal nitrite reductase inducible promoter having at least 90% sequence identity to SEQ ID NO:
- 1 operably linked to a DNA of interest encoding a polypeptide or a functional RNA, wherein the DNA of interest encoding the polypeptide or a functional RNA is not regulated by the promoter in nature, wherein the DNA of interest encodes (a) a protein associated with lipid biosynthesis, (b) a lipase, (c) a protein that participates in photosynthesis, (d) a protein associated with carbon fixation, (e) a transporter protein, (f) a dehydrogenase, (g) a transcription factor, (h) a transcriptional activator, (i) a cell signaling protein, (j) a metabolic enzyme, (k) a reporter protein, (l) a selectable marker, (m) a recombinase, n) an antisense sequence, (o) a shRNA, (p) an siRNA, (q) a gRNA, or (r) a ribozyme.
A method of selectively expressing a DNA of interest in an algal cell comprising:
- a) transforming an algal cell with an isolated DNA molecule comprising an algal nitrite reductase inducible promoter having at least 90% sequence identity to SEQ ID NO:
- 1 operably linked to a DNA of interest encoding the DNA of interest, wherein the DNA of interest encoding the DNA of interest is not regulated by the promoter in nature to generate transformed algal cells;
- b) growing the transformed algal cells in a media that selectively permits the expression of the DNA of interest in the algal cell.
Dependencies are inferred from the claim text.