US 11,162,080 B2 · Attenuated viruses useful for vaccines

Abstract

This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

Claims6

Dependent claims

A method of making a modified viral genome comprising:

a) obtaining the nucleotide sequence of a protein encoding region of a parent virus;
b) substituting a plurality of codons of all or part of the nucleotide sequence with synonymous codons that are less frequently used in a host to obtain a mutated nucleotide sequence that
i) encodes the same amino acid sequence as the protein encoding region of the parent virus, and
ii) comprises a plurality of codons that are less frequently used in the host compared to the synonymous codons in the nucleotide sequence of the protein encoding region of the parent virus;
and
c) substituting all or part of the mutated nucleotide sequence into the sequence of the parent virus.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

16/036,348Filed 16 Jul 2018 · continuation

Priority

30 Mar 2007

Published

US 2019/0010469 A110 Jan 2019

Granted

2 Nov 2021

Term ends

Not estimatedThe source records don’t support an estimate.

Classification36 codes

C12N 7/00
Viruses
A61K 39/12
Medicinal preparations containing antigens or antibodies: viral antigens
A61K 39/13
Picornaviridae, e.g. calicivirus: poliovirus
A61K 39/145
Viral antigens: orthomyxoviridae, e.g. influenza virus

Family

Application60/909,389Us provisional application · filed 30 Mar 2007

Application61/068,666Us provisional application · filed 7 Mar 2008

PCT/US2008/058952Parent of this division · filed 31 Mar 2008

Application15/258,584Parent of this continuation · filed 7 Sep 2016

US 9,476,032 B2Parent of this division · filed 25 Oct 2016

US 10,023,845 B2Parent of this continuation · filed 17 Jul 2018

US 2019/0010469 A1Pre-grant publication · published 10 Jan 2019

Application12/594,173Parent of this division

Application15/258,584Parent of this division

US 2010/0209454 A1Published application

US 2017/0067030 A1Published application

US 11,162,080 B2This patent

From the US filing history. Foreign family members aren’t listed.

Attenuated viruses useful for vaccines

Life of this patent

Attenuated viruses useful for vaccines

Life of this patent

Abstract

Claims6