Method of detecting an endotoxin using limulus amebocyte lysate substantially free of coagulogen
AssigneeLonza Walkersville, Inc.
InventorsCandice Stumbaugh, David S. Herbst, Kenneth E. Nichols, Jr.
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Abstract
The present invention is related to a method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising: (a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate; and (b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample; wherein the LAL is substantially free of coagulogen. The method also relates to compositions and kits comprising LAL substantially free of coagulogen, and methods of making such.
Claims13
A method of detecting an endotoxin in a sample using a chromogenic assay, the method comprising:
- a) contacting the sample with a reagent comprising limulus amebocyte lysate (LAL) and a chromogenic substrate;
- b) measuring a chromogenic effect resulting from a change in the chromogenic substrate in the presence of endotoxin in the sample;
- wherein the LAL has had a portion of coagulogen removed by filtration.
A method of detecting an endotoxin in a biological sample using a chromogenic assay, the method comprising:
- a) contacting the biological sample with an aqueous reagent comprising limulus amebocyte lysate (LAL) and Ac-Ile-Glu-Ala-Arg-pNA (SEQ ID NO:1);
- b) measuring the change in absorbance at 405 nm resulting from the enzymatic cleavage of pNA from Ac-Ile-Glu-Ala-Arg-pNA (SEQ ID NO:1) in the presence of endotoxin in the sample;
- wherein the LAL has had a portion of coagulogen removed by filtration.
Dependencies are inferred from the claim text.